Anti-beta Catenin antibody [9F2]

• 产品名称

  Anti-beta Catenin抗体[9F2]
  参阅全部 beta Catenin 一抗

• 描述

  小鼠单克隆抗体[9F2] to beta Catenin

• 宿主

  Mouse

• 特异性

  This antibody detects beta catenin from Human samples.

• 经测试应用

  适用于: WB, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant fragment (MBP-tag) corresponding to Human beta Catenin.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: PBS, 0.1% BSA

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  Immunogen purified on amylose column.

• 克隆

  单克隆

• 克隆编号

  9F2

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab19381于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1499 Human

• Omim: 116806 Human

• SwissProt: P35222 Human

• Unigene: 476018 Human

• 别名

  hide

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• b-catenin antibody

• Beta catenin antibody

• Beta-catenin antibody

• 

  Western blot - Anti-beta Catenin antibody [9F2] (ab19381)

  All lanes : Anti-beta Catenin antibody [9F2] (ab19381) at 3 µg/ml
  
  Lane 1 : HeLa whole cell lysate
  Lane 2 : U-97 MG whole cell lysate
  Lane 3 : COLO 205 whole cell lysate
  Lane 4 : HCT 116 whole cell lysate
  Lane 5 : HT-29 whole cell lysate
  Lane 6 : MCF7 whole cell lysate
  Lane 7 : MDA-MB-231 whole cell lysate
  Lane 8 : A549 whole cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution
  
  Predicted band size: 85 kDa
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [9F2] (ab19381)

  Immunofluorescent analysis of Beta-Catenin using Beta-Catenin Monoclonal antibody (9F2) ab19381 shows staining in HeLa cells. Beta-Catenin staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Beta-Catenin ab19381 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody [9F2] (ab19381)

  Immunofluorescent analysis of Beta-Catenin using Beta-Catenin Monoclonal antibody (9F2) ab19381 shows staining in U251 glioma cells. Beta-Catenin staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Beta-Catenin ab19381 at a dilution of 1:20 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.