Anti-EIF3C抗体
参阅全部 EIF3C 一抗
兔多克隆抗体to EIF3C
Rabbit
适用于: WB, IPmore details
与反应: Human
预测可用于: Rat, Cow, Orangutan
Synthetic peptide corresponding to Human EIF3C aa 850-913 (C terminal).
WB: HeLa and HEK293T cell lysates IP: HEK293T cell lysate.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab19360于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/15000 - 1/30000. Detects a band of approximately 98 kDa (predicted molecular weight: 105 kDa). | |
IP | Use at an assay dependent concentration. |
Entrez Gene: 8663 Human
Omim: 603916 Human
SwissProt: Q99613 Human
Unigene: 567374 Human
Unigene: 4207 Rat
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Western blot - Anti-EIF3C antibody (ab19360)
All lanes : Anti-EIF3C antibody (ab19360) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 105 kDa
Exposure time: 3 minutes
Detection: Chemiluminescence
Immunoprecipitation - Anti-EIF3C antibody (ab19360)
ab19360 immunoprecipitating EIF3C in HEK293T whole cell lysate.
Lane 1 : Rabbit anti EIF3C + HEK293T whole cell lysate
Lane 2 (+): ab19360 + HEK293T whole cell lysate.
Lane 3 (-): Control IgG instead of ab19360 in HEK293T whole cell lysate.
For western blotting, ab19360 was used at 1µg/mL.
Detection: Chemiluminescence