Anti-P cadherin抗体[6A9]
参阅全部 P cadherin 一抗
小鼠单克隆抗体[6A9] to P cadherin
Mouse
适用于: ICC/IF, ICCmore details
与反应: Human
Full length protein corresponding to P cadherin. Proteins removed from A431 cell membranes by trypsinization.
ICC: A431 cells. ICC/IF: A431 cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Affinity purified
单克隆
6A9
IgG1
kappa
Abpromise™承诺保证使用ab19350于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| ICC | Use a concentration of 5 µg/ml. |
Entrez Gene: 1001 Human
Omim: 114021 Human
SwissProt: P22223 Human
Unigene: 191842 Human
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![Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (ab19350)](https://www.abcam.cn/ps/products/19/ab19350/Images/ab19350-339377-ICCAP439715901.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (ab19350)
ab19350 staining P-Cadherin in A431 cells (top panel) and MCF7 (negative cell line - bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19350 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (ab19350)](https://www.abcam.cn/ps/products/19/ab19350/Images/ab19350-319891-ICCAP439715901.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (ab19350)
ab19350 staining P-Cadherin in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19350 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
