Anti-N Cadherin antibody [8C11]

• 产品名称

  Anti-N Cadherin抗体[8C11]
  参阅全部 N Cadherin 一抗

• 描述

  小鼠单克隆抗体[8C11] to N Cadherin

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IHC-Pmore details

• 种属反应性

  与反应: Human, Bird
  预测可用于: Rabbit, Hamster不与反应: Mouse, Rat, Cow, Pig

• 免疫原

  Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

• 表位

  The 8C11 monoclonal binds to the extracellular domain of N-cadherin between EC3 and EC4 (PubMed ID: 12604612).

• 阳性对照

• IHC-P: Normal human heart tissue sections. ICC/IF: SH-SY5Y cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  8C11

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab19348于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1000 Human

• Omim: 114020 Human

• SwissProt: P19022 Human

• Unigene: 464829 Human

• Unigene: 606106 Human

• 别名

• Cadherin 2 type 1 antibody

• Cadherin 2 type 1 N cadherin neuronal antibody

• Cadherin 2, type 1, N-cadherin (neuronal) antibody

• Cadherin-2 antibody

• Cadherin2 antibody

• Calcium dependent adhesion protein neuronal antibody

• CD325 antibody

• CD325 antigen antibody

• CDH2 antibody

• CDHN antibody

• CDw325 antibody

• CDw325 antigen antibody

• N cadherin 1 antibody

• N-cadherin antibody

• NCAD antibody

• Neural cadherin antibody

• OTTHUMP00000066304 antibody

• OTTHUMP00000067378 antibody

• CADH2_HUMAN antibody

• Cadherin 2 antibody

• Cadherin 2 N cadherin neuronal antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [8C11] (ab19348)

  IHC image of N cadherin staining in a section of formalin-fixed paraffin-embedded normal human heart performed on a Leica BONDTM system using the standard protocol F.

  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19348, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

  The inset secondary-only control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (ab19348)

  ab19348 staining N-Cadherin in SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells.

  The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19348 at 5 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (ab19348)Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9. Fig 1.; doi: 10.1371/journal.pone.0036864; May 9, 2012, PLoS One. 2012; 7(5): e36864. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunofluorescence analysis of ARPE-19 (human retinal pigment epithelial) cells, staining N Cadherin (green) with ab19348, at 1/20 dilution.
  
  ARPE-19 monolayer cultures were fixed in paraformaldehyde, permeabilized with 0.2% Triton X-100 for 15 min and blocked with 2% BSA for 30 min. Samples were incubated with primary antibody for 16 hours at 4°C before incubation with an Alexa Fluor® 488-conjugated donkey anti-mouse secondary IgG for 60 min.