Anti-CaSR antibody [5C10, ADD]

• 产品名称

  Anti-CaSR抗体[5C10，ADD]
  参阅全部 CaSR 一抗

• 描述

  小鼠单克隆抗体[5C10，ADD] to CaSR

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, Flow Cyt, IHC-Frmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide within Human CaSR aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

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• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: PBS, 0.1% BSA

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  单克隆

• 克隆编号

  5C10, ADD

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab19347于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 846 Human

• Entrez Gene: 12374 Mouse

• Omim: 601199 Human

• SwissProt: P41180 Human

• SwissProt: Q9QY96 Mouse

• Unigene: 435615 Human

• Unigene: 103619 Mouse

• 别名

• Calcium sensing receptor antibody

• CAR antibody

• CaSR antibody

• CASR_HUMAN antibody

• EIG8 antibody

• Extracellular calcium sensing receptor antibody

• Extracellular calcium sensing receptor [Precursor] antibody

• Extracellular calcium-sensing receptor antibody

• FHH antibody

• FIH antibody

• GPRC2A antibody

• HHC antibody

• HHC1 antibody

• Hypocalciuric hypercalcemia 1 antibody

• Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody

• MGC138441 antibody

• NSHPT antibody

• Parathyroid Ca(2+) sensing receptor 1 antibody

• Parathyroid Cell calcium sensing receptor antibody

• Parathyroid Cell calcium-sensing receptor antibody

• PCAR 1 antibody

• PCaR1 antibody

• Ca sensing receptor antibody

• Ca2+ sensing receptor 1 antibody

• Ca2+ sensing receptor antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)

  Immunohistochemical analysis of 4% formalin fixed frozen mouse stomach tissue labeling CaSR with ab19347 at 1/100 dilution overnight at 4°C, followed by fluorophore-conjugated goat anti-mouse IgG secondary antibody for 2 hours at RT.

  Fresh stomach tissue was fixed in 4% formalin for 1 hour and then incubated overnight at  in 25% sucrose before embedding in tissue freezing medium. Antigen retrieval was carried out on 8µm cryo-sections by incubating in sodium citrate buffer for 45 minutes at 4°C, immersing in sodium citrate buffer for 10 minutes at 100°C before washing 3 times for 5 minutes each in 1X PBS. Sections were then blocked in blocking buffer (0.3% Triton X-100 in 1X PBS containing 10% normal goat serum) for 30 minutes at RT before staining with ab19347.

  Sections were also stained with DAPI nuclear stain (blue). Positive cells (green) were found at the base of the antral glands in the mouse stomach.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing CaSR ab19347 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

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  Flow Cytometry - Anti-CaSR antibody [5C10, ADD] (ab19347)

  Overlay histogram showing SH-SY5Y cells stained with ab19347 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19347, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing CaSR ab19347 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

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  Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)This image is courtesy of an anonymous Abreview

  ab19347 at 1/100 staining rat brain (cerebral cortex) tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum before incubation with the primary antibody for 24 hours at 4°C. A biotinylated horse anti-mouse IgG was used as the secondary.

  See Abreview