HRP Anti-c-Myc antibody

• 产品名称

  HRP Anti-c-Myc抗体
  参阅全部 c-Myc 一抗

• 描述

  HRP兔多克隆抗体to c-Myc

• 宿主

  Rabbit

• 偶联物

  HRP

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Human, Recombinant fragment
  

• 免疫原

  Synthetic peptide corresponding to Human c-Myc aa 410-419 conjugated to keyhole limpet haemocyanin.
  Sequence:

  EQKLISEEDL

  
  Database link: P01106

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  pH: 6.8
  Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab19312于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4609 Human

• Omim: 190080 Human

• SwissProt: P01106 Human

• Unigene: 202453 Human

• 形式

  c-Myc is also expressed in the cytoplasm.

• 别名

• c Myc antibody

• Cellular myelocytomatosis oncogene antibody

• Class E basic helix-loop-helix protein 39 antibody

• MGC105490 antibody

• MRTL antibody

• Myc antibody

• Myc protein antibody

• Myc proto oncogene protein antibody

• Myc proto-oncogene protein antibody

• myc-related translation/localization regulatory factor antibody

• MYC_HUMAN antibody

• Myc2 antibody

• myca antibody

• MYCC antibody

• Myelocytomatosis oncogene a antibody

• Myelocytomatosis oncogene antibody

• Niard antibody

• Nird antibody

• oncogene c-Myc antibody

• Oncogene Myc antibody

• OTTHUMP00000158589 antibody

• OTTHUMP00000227763 antibody

• Proto-oncogene c-Myc antibody

• Protooncogene homologous to myelocytomatosis virus antibody

• RNCMYC antibody

• Transcription factor p64 antibody

• Transcriptional regulator Myc-A antibody

• V-Myc avian myelocytomatosis viral oncogene homolog antibody

• v-myc myelocytomatosis viral oncogene homolog (avian) antibody

• zc-myc antibody

• AU016757 antibody

• Avian myelocytomatosis viral oncogene homolog antibody

• bHLHe39 antibody

• 

  Immunocytochemistry/ Immunofluorescence - HRP Anti-c-Myc antibody (ab19312)

  ICC/IF image of ab19312 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19312, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

• 

  Western blot - HRP Anti-c-Myc antibody (ab19312)

  All lanes : HRP Anti-c-Myc antibody (ab19312) at 0.4 µg/ml
  
  Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
  Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
  Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
  
  Predicted band size: 49 kDa
  
  
  Exposure time: 10 seconds
  
  
  

  Detection: Chemiluminesence