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HRP Anti-T7 tag® antibody

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  • 产品名称

    HRP Anti-T7 tag®抗体
    参阅全部 T7 tag® 一抗

  • 描述

    HRP兔多克隆抗体to T7 tag®

  • 宿主

    Rabbit

  • 偶联物

    HRP

  • 经测试应用

    适用于: ELISAICCWBmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Synthetic peptide:

    MASMTGGQQMG

    conjugated to KLH.

    Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

  • 常规说明

    Molar enzyme/antibody protein ratio is 4:1.


    T7-TAG® is a trademark of EMD Biosciences, Inc.


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 6.8
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA

  • 浓度

    • 100 µg 浓度为 0.5 mg/ml

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP).

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab19291于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
ELISA

ICC

WB

应用说明

ELISA: 1/10000 - 1/100000.
ICC: 1/200 - 1/500.
WB: 1/1000 - 1/30000. Colorimetric detection: 1:1000 - 1:10000, Chemiluminescent detection: 1:1000 - 1:30000

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

别名

  • 10 antibody

  • Major capsid protein 10A antibody

  • Major capsid protein antibody

  • Minor capsid protein 10B antibody

  • T7 epitope tag antibody

  • Western blot - HRP Anti-T7 tag® antibody (ab19291)

    Western blot - HRP Anti-T7 tag® antibody (ab19291)

    All lanes : HRP Anti-T7 tag® antibody (ab19291) at 1/5000 dilution

    Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
    Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
    Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

    Predicted band size: 37 kDa


    Exposure time: 3 minutes


    Detected by chemiluminescence.


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