HRP Anti-T7 tag®抗体
参阅全部 T7 tag® 一抗
HRP兔多克隆抗体to T7 tag®
Rabbit
HRP
适用于: ELISA, ICC, WBmore details
与反应: Species independent
Synthetic peptide:
MASMTGGQQMG
conjugated to KLH.
Molar enzyme/antibody protein ratio is 4:1.
T7-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Liquid
Shipped at 4°C. Store at +4°C.
pH: 6.8
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP).
多克隆
IgG
Abpromise™承诺保证使用ab19291于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA | ||
ICC | ||
WB |
应用说明
ELISA: 1/10000 - 1/100000.
ICC: 1/200 - 1/500.
WB: 1/1000 - 1/30000. Colorimetric detection: 1:1000 - 1:10000, Chemiluminescent detection: 1:1000 - 1:30000
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
10 antibody
Major capsid protein 10A antibody
Major capsid protein antibody
Minor capsid protein 10B antibody
T7 epitope tag antibody
Western blot - HRP Anti-T7 tag® antibody (ab19291)
All lanes : HRP Anti-T7 tag® antibody (ab19291) at 1/5000 dilution
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Predicted band size: 37 kDa
Exposure time: 3 minutes
Detected by chemiluminescence.
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