Anti-Enterokinase cleavage site (xxxDDDDK)抗体
兔多克隆抗体to Enterokinase cleavage site (xxxDDDDK)
Rabbit
By ELISA, western blot and immunofluorescence, this antibody reacts with FLAG.
适用于: ELISA, WB, ICC/IFmore details
与反应: Species independent
Recombinant fragment corresponding to Rabbit Enterokinase cleavage site (xxxDDDDK). Rabbits were immunized with ECS, enterokinase cleavage site (xxxDDDDK) conjugated to KLH.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 6.8
Preservative: 0.1% Sodium azide
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab19231于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA | ||
WB | ||
ICC/IF |
应用说明
ELISA: Coating: 1/100-1/500
Primary: 1/1000-1/30,000
ICC: 1/100 - 1/400.
WB: Colorimetric detection 1/1,000-1/10,000
Chemiluminescence 1/1,000-1/30,000. Predicted molecular weight: 20 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ECS antibody
enterokinase antibody
xxxDDDDK antibody
Western blot - Anti-Enterokinase cleavage site (xxxDDDDK) antibody (ab19231)
All lanes : Anti-Enterokinase cleavage site (xxxDDDDK) antibody (ab19231) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Predicted band size: 20 kDa
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