Anti-NFkB Inducing Kinase NIK抗体
参阅全部 NFkB Inducing Kinase NIK 一抗
兔多克隆抗体to NFkB Inducing Kinase NIK
Rabbit
This antibody recognises human NFkB Inducing Kinase NIK.
适用于: IHC-P, ICC/IF, WBmore details
与反应: Human
Synthetic peptide:
SFAWSWRVKHGQLENRP
, corresponding to amino acids 931-947 of Human NFkB Inducing Kinase NIK
Whole cell lysate of 293.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab19144于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 5 µg/ml. | |
| WB | 1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 105.4 kDa). |
Entrez Gene: 9020 Human
Omim: 604655 Human
SwissProt: Q99558 Human
Unigene: 404183 Human
Unigene: 668927 Human
Unigene: 735695 Human
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Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab19144)
Anti-NFkB Inducing Kinase NIK antibody (ab19144) at 1/500 dilution + NIK-transfected 293 cell lysate
Predicted band size: 105.4 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (ab19144)
ICC/IF image of ab19144 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19144, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB Inducing Kinase NIK antibody (ab19144)
IHC image of ab19144 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19144, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
