Human Tau (phospho S214) peptide

• 产品名称

  人Tau (phospho S214)多肽
  参阅全部 Tau 蛋白酶

• 纯度

  > 70 % HPLC.
  70 - 90% by HPLC
  

• 
  

• 无动物成分

  No

• 性质

  Synthetic

• 
  

• 种属

  Human

• 修饰

  phospho S214

Our Abpromise guarantee covers the use of ab19123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  Blocking

• 形式

  Lyophilized

• 补充说明

  - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
  - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
  - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
  - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
  - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

• 稳定性和存储

  Shipped at 4°C. Store at -20°C.

  Information available upon request.

别名

• AI413597

• AW045860

• DDPAC

• FLJ31424

• FTDP 17

• G protein beta1/gamma2 subunit interacting factor 1

• MAPT

• MAPTL

• MGC134287

• MGC138549

• MGC156663

• Microtubule associated protein tau

• Microtubule associated protein tau isoform 4

• Microtubule-associated protein tau

• MSTD

• Mtapt

• MTBT1

• MTBT2

• Neurofibrillary tangle protein

• Paired helical filament tau

• Paired helical filament-tau

• PHF tau

• PHF-tau

• PPND

• PPP1R103

• Protein phosphatase 1, regulatory subunit 103

• pTau

• RNPTAU

• TAU

• TAU_HUMAN

• Tauopathy and respiratory failure

• Tauopathy and respiratory failure, included

• 

  ELISA - Human Tau (phospho S214) peptide (ab19123)

  ab10891 gave a positive result in ELISA against the immunising peptide (ab19123). This ELISA shows that ab10891 antibody has some cross-reactivity with the non-modified equivalent peptide (ab23425) when used at high concentrations. Not yet tested in other applications.

• 

  Western blot - Human Tau (phospho S214) peptide (ab19123)

  All lanes : Anti-Tau (phospho S214) antibody (ab10891) at 1/250 dilution
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Tau (phospho S214) peptide (ab19123) at 1 µg/ml
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Exposure time: 2 minutes
  
  
  Tau contains a number of potential phosphorylation and glycosylation sites (Swiss Prot data) which may explain its migration at a higher molecular weight than predicted.