Anti-Androgen Receptor抗体
参阅全部 Androgen Receptor 一抗
山羊多克隆抗体to Androgen Receptor
Goat
适用于: WB, IHC-Pmore details
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Pig, Chimpanzee
Synthetic peptide:
EVQLGLGRVYPRPPSC
, corresponding to N terminal amino acids 2-16 of Human Androgen Receptor
Human Brain and Human Heart lysates.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab19066于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.3 - 2 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). A 1 hour primary incubation is recommended for this product. | |
| IHC-P | Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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SwissProt: O97775 Chimpanzee
SwissProt: P10275 Human
SwissProt: P19091 Mouse
Unigene: 76704 Human
Unigene: 39005 Mouse
Unigene: 394224 Mouse
Unigene: 439657 Mouse
Unigene: 9813 Rat
Entrez Gene: 747460 Chimpanzee
Entrez Gene: 367 Human
Entrez Gene: 11835 Mouse
Omim: 313700 Human
There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody (ab19066)
ab19066 (2µg/ml) staining of paraffin embedded Human Prostate following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows nuclear staining in the secretory cells of the gland.
Western blot - Anti-Androgen Receptor antibody (ab19066)
ab19066 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer) approx. 99kDa. Primary incubation was 1 hour. Detected by chemiluminescence.
ab19066 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer) approx. 99kDa. Primary incubation was 1 hour. Detected by chemiluminescence.
