Anti-WRNIP1/WHIP抗体
参阅全部 WRNIP1/WHIP 一抗
山羊多克隆抗体to WRNIP1/WHIP
Goat
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog
Synthetic peptide corresponding to Human WRNIP1/WHIP aa 652-665 (C terminal).
Sequence:
EELRGVDFFKQRRC
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab18729于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 72 kDa. 1 hour primary incubation is recommended for this product. |
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Unigene: 286680 Mouse
Unigene: 8783 Rat
Entrez Gene: 56897 Human
Entrez Gene: 78903 Mouse
Omim: 608196 Human
SwissProt: Q96S55 Human
SwissProt: Q91XU0 Mouse
Unigene: 236828 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WRNIP1/WHIP antibody (ab18729)
ab18729 (4µg/ml) staining of paraffin embedded Human Kidney following steamed antigen retrieval with Tris/EDTA buffer pH 9 and HRP-staining shows nuclear staining, stronger in DCT compared to PCT.
Western blot - Anti-WRNIP1/WHIP antibody (ab18729)
Anti-WRNIP1/WHIP antibody (ab18729) at 1 µg/ml + Jurkat Cell Lysate in RIPA Buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
