Anti-Sialoadhesin/CD169 antibody [HSn 7D2]

• 产品名称

  Anti-Sialoadhesin/CD169抗体[HSn 7D2]
  参阅全部 Sialoadhesin/CD169 一抗

• 描述

  小鼠单克隆抗体[HSn 7D2] to Sialoadhesin/CD169

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-Frmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length native protein (purified) corresponding to Human Sialoadhesin/CD169.

• 表位

  Within domains 1-4.

• 阳性对照

• WB: Human spleen and human lymph node whole tissue lysate. IHC-Fr: Human liver normal tissue.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  HSn 7D2

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab18619于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 6614 Human

• Omim: 600751 Human

• SwissProt: Q9BZZ2 Human

• Unigene: 31869 Human

• 别名

• DKFZp667F058 antibody

• FLJ00051 antibody

• FLJ00055 antibody

• FLJ00073 antibody

• FLJ00411 antibody

• FLJ32150 antibody

• Sialic acid binding Ig like lectin 1 antibody

• sialic acid binding Ig-like lectin 1, sialoadhesin antibody

• Sialic acid binding immunoglobulin like lectin 1 antibody

• Sialic acid-binding Ig-like lectin 1 antibody

• Sialoadhesin antibody

• Siglec 1 antibody

• Siglec-1 antibody

• Siglec1 antibody

• SN antibody

• SN_HUMAN antibody

• CD169 antibody

• CD169 antigen antibody

• dJ1009E24.1 antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] (ab18619)

  IHC image of Sialoadhesin/CD169 staining in human liver frozen tissue section, fixed in 10% paraformaldehyde (10 min). Staining was performed on a Leica Bond system using the standard protocol F. The section was then incubated with ab18619, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Western blot - Anti-Sialoadhesin/CD169 antibody [HSn 7D2] (ab18619)

  All lanes : Anti-Sialoadhesin/CD169 antibody [HSn 7D2] - BSA and Azide free (ab269566) at 1 µg/ml
  
  Lane 1 : Human spleen whole tissue lysate
  Lane 2 : Human lymph node whole tissue lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Exposure time: 20 minutes
  
  
  

  This blot was produced using a 3-8% Tris-Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes.

  The membrane was then blocked for an hour using 2% BSA before being incubated with ab269566 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  This image was produced using ab269566, the same antibody clone but in a different PBS only formulation