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Anti-Cytokeratin 6 antibody [Ks6.KA12]

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500uL
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产品详情
  • 产品名称

    Anti-Cytokeratin 6抗体[Ks6.KA12]
    参阅全部 Cytokeratin 6 一抗

  • 描述

    小鼠单克隆抗体[Ks6.KA12] to Cytokeratin 6

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    A cytoskeletal preparation of human callus material was used for immunization.

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • 存储溶液

    pH: 7.3
    Preservative: 0.09% Sodium azide
    Constituents: 0.5% BSA, PBS

  • 纯度

    Protein A purified

  • 纯化说明

    Purified by protein A affinity chromatography.

  • 克隆

    单克隆

  • 克隆编号

    Ks6.KA12

  • 同种型

    IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab18586于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
Flow Cyt

Use 2µg for 106 cells. 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

  • 数据库链接

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  • 别名

    • CK 6D antibody

    • CK 6E antibody

    • CK-6B antibody

    • CK-6C antibody

    • CK-6E antibody

    • Cytokeratin 6a antibody

    • Cytokeratin 6B antibody

    • Cytokeratin 6C antibody

    • Cytokeratin 6D antibody

    • Cytokeratin 6E antibody

    • Cytokeratin-6B antibody

    • Cytokeratin-6C antibody

    • Cytokeratin-6E antibody

    • K2C6C_HUMAN antibody

    • K6a keratin antibody

    • K6b keratin antibody

    • K6C antibody

    • K6c keratin antibody

    • K6d keratin antibody

    • K6e keratin antibody

    • Keratin antibody

    • Keratin K6h antibody

    • Keratin type II cytoskeletal 6A antibody

    • Keratin type II cytoskeletal 6B antibody

    • Keratin type II cytoskeletal 6C antibody

    • Keratin type II cytoskeletal 6D antibody

    • Keratin type II cytoskeletal 6E antibody

    • Keratin-6C antibody

    • KRT6A antibody

    • KRT6B antibody

    • KRT6C antibody

    • KRT6D antibody

    • KRT6E antibody

    • type II cytoskeletal 6C antibody

    • Type-II keratin Kb12 antibody

    • CK 6A antibody

    • CK 6B antibody

    • CK 6C antibody

Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (ab18586)

Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (ab18586)

Overlay histogram showing A431 cells stained with ab18586 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18586, 2µg/1x106 cells ) for 30 min at 22°C. The secondary antibody used was a goat anti-mous DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.