Anti-Cytokeratin 6抗体[Ks6.KA12]
参阅全部 Cytokeratin 6 一抗
小鼠单克隆抗体[Ks6.KA12] to Cytokeratin 6
Mouse
适用于: Flow Cytmore details
与反应: Human
A cytoskeletal preparation of human callus material was used for immunization.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: 0.5% BSA, PBS
Protein A purified
Purified by protein A affinity chromatography.
单克隆
Ks6.KA12
IgG1
Abpromise™承诺保证使用ab18586于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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SwissProt: P48668 Human
Unigene: 700779 Human
Unigene: 709234 Human
Entrez Gene: 286887 Human
Entrez Gene: 3853 Human
Entrez Gene: 3854 Human
Omim: 148041 Human
Omim: 148042 Human
Omim: 612315 Human
SwissProt: P02538 Human
SwissProt: P04259 Human
CK 6D antibody
CK 6E antibody
CK-6B antibody
CK-6C antibody
CK-6E antibody
Cytokeratin 6a antibody
Cytokeratin 6B antibody
Cytokeratin 6C antibody
Cytokeratin 6D antibody
Cytokeratin 6E antibody
Cytokeratin-6B antibody
Cytokeratin-6C antibody
Cytokeratin-6E antibody
K2C6C_HUMAN antibody
K6a keratin antibody
K6b keratin antibody
K6C antibody
K6c keratin antibody
K6d keratin antibody
K6e keratin antibody
Keratin antibody
Keratin K6h antibody
Keratin type II cytoskeletal 6A antibody
Keratin type II cytoskeletal 6B antibody
Keratin type II cytoskeletal 6C antibody
Keratin type II cytoskeletal 6D antibody
Keratin type II cytoskeletal 6E antibody
Keratin-6C antibody
KRT6A antibody
KRT6B antibody
KRT6C antibody
KRT6D antibody
KRT6E antibody
type II cytoskeletal 6C antibody
Type-II keratin Kb12 antibody
CK 6A antibody
CK 6B antibody
CK 6C antibody
Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (ab18586)
Overlay histogram showing A431 cells stained with ab18586 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18586, 2µg/1x106 cells ) for 30 min at 22°C. The secondary antibody used was a goat anti-mous DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.