Anti-LAMP2B抗体- Lysosome Marker
参阅全部 LAMP2B 一抗
兔多克隆抗体to LAMP2B - Lysosome Marker
Rabbit
This product has low sequence homology with Mouse LAMP2B (67%), and high sequence homology with Mouse LAMP2C (91.7%).
适用于: WBmore details
与反应: Human
预测可用于: Cow
Synthetic peptide corresponding to Human LAMP2B aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. LAMP2 has 3 distinct isoforms, LAMP2A, 2B & 2C which are expressed in different tissues and differ in sequence at the extreme C-terminus.
Database link: P13473
(Peptide available as ab23321)
This antibody gave a positive signal in the following whole cell lysates: HeLa, HEK293. This antibody gave a positive signal in the following Methanol fixed cell lines: HeLa. It also gave a positive signal in Human skin tissue sections.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab18529于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
Entrez Gene: 3920 Human
Omim: 309060 Human
SwissProt: P13473 Human
Unigene: 496684 Human
Alternative splicing produces 3 isoforms.
CD107b antibody
LAMP 2 antibody
Lamp 2b antibody
LAMP-2 antibody
LAMP2 antibody
LAMP2_HUMAN antibody
LAMPB antibody
LGP110 antibody
Lysosomal associated membrane protein 2 antibody
Lysosome associated membrane glycoprotein 2 antibody
Lysosome-associated membrane glycoprotein 2 antibody
Lysosome-associated membrane protein 2 antibody
MAC3 antibody
CD_antigen=CD107b antibody
CD107 antigen like family member B antibody
CD107 antigen-like family member B antibody
Western blot - Anti-LAMP2B antibody - Lysosome Marker (ab18529)
All lanes : Anti-LAMP2B antibody - Lysosome Marker (ab18529) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Additional bands at: 22 kDa (possible non-specific binding), 33 kDa (possible non-specific binding), 34 kDa (possible non-specific binding), 65 kDa (possible non-specific binding)
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The blot was incubated with ab18529 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
LAMP2B undergoes extensive glycosylation so ab18529 detects a band of 110 kDa by Western blot.
