Human RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide

• 产品名称

  人RNA polymerase II CTD repeat YSPTSPS (phospho S5)多肽
  参阅全部 RNA polymerase II CTD repeat YSPTSPS 蛋白酶

• 纯度

  > 90 % HPLC.
  
  

• Accession

  P24928

• 无动物成分

  No

• 性质

  Synthetic

• 
  

• 种属

  Human

• 修饰

  phospho S1675 + S1682

Our Abpromise guarantee covers the use of ab18488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  Western blot

  Blocking - Blocking peptide for Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131)

• 形式

  Lyophilized

• 补充说明

  This peptide is 2 repeats of the YSPTSPS repeated motif found at the C-terminal of RNA polymerase II.

  - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
  - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
  - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
  - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
  - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

• 稳定性和存储

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  Information available upon request.

• 复溶

  Reconstitute with 100 ul of deionised water (or equivalent).

别名

• DNA directed RNA polymerase II A

• DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit

• DNA-directed RNA polymerase II subunit A

• DNA-directed RNA polymerase II subunit RPB1

• DNA-directed RNA polymerase III largest subunit

• hRPB220

• hsRPB1

• POLR2

• Polr2a

• POLRA

• Polymerase (RNA) II (DNA directed) polypeptide A

• Polymerase (RNA) II (DNA directed) polypeptide A 220kDa

• RNA polymerase II subunit B1

• RNA-directed RNA polymerase II subunit RPB1

• RPB1

• RPB1_HUMAN

• RPBh1

• RpIILS

• RPO2

• RPOL2

• 

  Dot Blot - Human RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide (ab18488)This image is courtesy of an anonymous Abreview

  Dot blot analysis of ab18488 - RNA polymerase II CTD repeat YSPTSPS peptide - phospho S5.
  
  0.1 µg peptide was spotted onto the membrane and blocked with 2% BSA for 1 hour at room temperature, before detection with ab5131 at 1/3000 dilution.

  See Abreview