大鼠IgG2a，kappa单克隆抗体[RTK2758] -同型对照
参阅全部 Rat 亚类对照
Please note that this antibody is unconjugated.
适用于: ICC/IF, Flow Cyt, IP, WB, IHC-P, IHC-Frmore details
Trinitrophenol + KLH
ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C. Do Not Freeze.
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS
0.2 µm filter sterilized
浓度
500 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
The immunoglobulin was purified by affinity chromatography.
ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
单克隆
RTK2758
IgG2a
kappa
Rat Isotype Control
Abpromise™承诺保证使用ab18450于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use 2µg for 106 cells. ab18450 is a negative isotype control. | |
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
![Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)](https://www.abcam.cn/ps/products/18/ab18450/Images/ab18450-28038-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-low-endotoxin-azide-free-flow-cytometry.jpg)
Flow Cytometry - Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
