小鼠IgG2a，Kappa单克隆抗体[MOPC-173] -Isotype Control - ChIP Grade
参阅全部 Mouse 亚类对照
This Balb/c myeloma derived clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.
适用于: IP, WB, IHC-Fr, IHC-P, Flow Cyt, ChIP/Chip, ChIPmore details
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Liquid
Shipped at 4°C. Store at +4°C.
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS
浓度
500 µg 浓度为 0.5 mg/ml
Protein A purified
单克隆
MOPC-173
IgG2a
kappa
Mouse Isotype Control
Abpromise™承诺保证使用ab18413于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| ChIP/Chip | Use at an assay dependent concentration. PubMed: 18636108 | |
| ChIP | Use at an assay dependent concentration. PubMed: 18636108 |
![ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)](https://www.abcam.cn/ps/products/18/ab18413/Images/ab18413-277417-mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-chip.jpg)
ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)Image from Niemantsverdriet M et al., PLoS One. 2012;7(12):e50815. Fig 5.; doi: 10.1371/journal.pone.0050815. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.
