Anti-FOXG1抗体
参阅全部 FOXG1 一抗
兔多克隆抗体to FOXG1
Rabbit
Replenishment batches of our polyclonal antibody, ab18259 are tested in IHC-P. Previous batches were additionally validated in WB. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab196868.
适用于: WB, IHC-Pmore details
与反应: Mouse, Rat, Human
预测可用于: Xenopus laevis
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.7 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab18259于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
| IHC-P | (2) | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
hide
SwissProt: Q9YHC5 Xenopus laevis
Unigene: 695962 Human
Unigene: 708841 Human
Unigene: 4704 Mouse
Unigene: 9864 Rat
Unigene: 416 Xenopus laevis
Entrez Gene: 2290 Human
Entrez Gene: 15228 Mouse
Entrez Gene: 373732 Xenopus laevis
Omim: 164874 Human
SwissProt: P55316 Human
SwissProt: Q60987 Mouse
BF2 antibody
Brain factor 1 antibody
Brain factor 2 antibody
FHKL antibody
FKH2 antibody
FKHL1 antibody
FKHL2 antibody
FKHL3 antibody
FKHL4 antibody
Forkhead box protein G1 antibody
Forkhead box protein G1A antibody
Forkhead box protein G1B antibody
Forkhead box protein G1C antibody
Forkhead like 1 antibody
Forkhead like 2 antibody
Forkhead like 3 antibody
Forkhead like 4 antibody
Forkhead-related protein FKHL1 antibody
Forkhead-related protein FKHL2 antibody
Forkhead-related protein FKHL3 antibody
FOXG1 antibody
FOXG1_HUMAN antibody
FOXG1A antibody
FOXG1B antibody
FOXG1C antibody
HBF 1 antibody
HBF G2 antibody
hBF-2 antibody
HBF2 antibody
HFK1 antibody
HFK2 antibody
HFK3 antibody
KHL2 antibody
Oncogene QIN antibody
QIN antibody
BF-1 antibody
BF-2 antibody
BF1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody - ChIP Grade (ab18259)
IHC image of FoxG1 staining in mouse brain E14 formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18259, 0.5 µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Staining revealed in telencephalon (but not diencephalon) as expected.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-FOXG1 antibody - ChIP Grade (ab18259)
All lanes : Anti-FOXG1 antibody (ab18259) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : E10 Mouse Embryo Brain Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lane 5 : Human spleen tissue lysate - total protein (negative control)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 155 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab18259 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Western blot - Anti-FOXG1 antibody - ChIP Grade (ab18259)
All lanes : Anti-FOXG1 antibody (ab18259) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human FOXG1 peptide (ab19644) at 1 µg/ml
Lane 5 : Brain (Mouse) Tissue Lysate with Human FOXG1 peptide (ab19644) at 1 µg/ml
Lane 6 : Brain (Rat) Tissue Lysate with Human FOXG1 peptide (ab19644) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 1 minute
ab18259 detects a band of ~50kDa in brain lysates. This band is blocked by addition of the immunizing peptide ab19644 which suggests that is specific for FOXG1.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18259 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody (ab18259)This image is courtesy of Vassiliki Fotaki, University of Edinburgh
ab18259 staining Foxg1 (1/50) in the telencephalon (but not the diencephalon) as expected. DAB-immunohistochemistry was performed on embryonic (E13.5) mouse brain (coronal paraffin-embedded sections) after microwave treatment with 10mM sodium citrate.
