Anti-PARK7/DJ1抗体
参阅全部 PARK7/DJ1 一抗
兔多克隆抗体to PARK7/DJ1
Rabbit
Replenishment batches of our polyclonal antibody, ab18257 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab76008.
适用于: IHC-P, ICC/IF, WBmore details
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Cow, Dog
Synthetic peptide corresponding to Human PARK7/DJ1 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab18659)
WB: HeLa, Jurkat, PC12 and NIH 3T3 whole cell lysates, mouse brain, liver, heart, kidney, pancreas, testis, skeletal muscle, spinal cord and ovary tissue lysates and rat brain, liver, heart and kidney tissue lysates. IHC-P: Human parathyroid tissue. ICC/IF: HeLa cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.9 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab18257于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | (4) | Use a concentration of 1 µg/ml. |
| WB | (5) | Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 20 kDa). |
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Unigene: 419640 Human
Unigene: 277349 Mouse
Unigene: 30105 Rat
Entrez Gene: 11315 Human
Entrez Gene: 57320 Mouse
Omim: 602533 Human
SwissProt: Q99497 Human
SwissProt: Q99LX0 Mouse
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Western blot - Anti-PARK7/DJ1 antibody (ab18257)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PARK7/DJ1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab18257 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab18257 was shown to specifically react with PARK/DJ1 in wild-type HAP1 cells. No band was observed when PARK/DJ1 knockout samples were used. Wild-type and PARK/DJ1 knockout samples were subjected to SDS-PAGE. ab18257 and ab8245 (loading control to GAPDH) were diluted to 1µg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
Western blot - Anti-PARK7/DJ1 antibody (ab18257)
All lanes : Anti-PARK7/DJ1 antibody (ab18257) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : Mouse pancreas tissue lysate - total protein (ab29363)
Lane 6 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 7 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 8 : Spinal Cord (Mouse) Tissue Lysate
Lane 9 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 10 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 11 : Brain (Rat) Tissue Lysate - normal tissue
Lane 12 : Liver (Rat) Tissue Lysate
Lane 13 : Heart (Rat) Tissue Lysate
Lane 14 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 24 kDawhy is the actual band size different from the predicted?
Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody (ab18257)
ab18257 staining PARK7/DJ1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab18257 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-PARK7/DJ1 antibody (ab18257)
All lanes : Anti-PARK7/DJ1 antibody (ab18257) at 1 µg/ml
Lane 1 : Jurkat lysate
Lane 2 : HeLa lysate
Lane 3 : 3T3 lysate
Lane 4 : Jurkat lysate with Human PARK7/DJ1 peptide (ab18659) at 1 µg/ml
Lane 5 : HeLa lysate with Human PARK7/DJ1 peptide (ab18659) at 1 µg/ml
Lane 6 : 3T3 lysate with Human PARK7/DJ1 peptide (ab18659) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (680) at 1/10000 dilution
Predicted band size: 20 kDa
Observed band size: 24 kDawhy is the actual band size different from the predicted?
Western blot - Anti-PARK7/DJ1 antibody
