Anti-Cyclin B2/CCNB2 antibody [X29.2]

• 产品名称

  Anti-Cyclin B2/CCNB2抗体[X29.2]
  参阅全部 Cyclin B2/CCNB2 一抗

• 描述

  小鼠单克隆抗体[X29.2] to Cyclin B2/CCNB2

• 宿主

  Mouse

• 特异性

  Reacts with most cyclin Bs.

• 经测试应用

  适用于: IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length protein corresponding to Xenopus laevis Cyclin B2/CCNB2.
  Database link: P13351

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 100 µg 浓度为 0.5 mg/ml

• 纯度

  Protein A/G purified

• 克隆

  单克隆

• 克隆编号

  X29.2

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab18250于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 9133 Human

• Omim: 602755 Human

• SwissProt: O95067 Human

• Unigene: 194698 Human

• 别名

• Cyclin B2 antibody

• G2 mitotic specific cyclin B2 antibody

• G2/mitotic specific cyclin B2 antibody

• G2/mitotic-specific cyclin-B2 antibody

• HsT17299 antibody

• MGC108931 antibody

• MGC140694 antibody

• ccnb2 antibody

• CCNB2_HUMAN antibody

• CycB2 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B2/CCNB2 antibody [X29.2] (ab18250)

  IHC image of ab18250 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18250, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

• 

  Flow Cytometry - Anti-Cyclin B2/CCNB2 antibody [X29.2] (ab18250)

  Overlay histogram showing HeLA cells stained with ab18250 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18250, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.