Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01]

• 产品名称

  Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[F-tag-01]
  参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗

• 描述

  小鼠单克隆抗体[F-tag-01] to DDDDK tag (Binds to FLAG® tag sequence)

• 宿主

  Mouse

• 经测试应用

  适用于: ICC, ICC/IFmore details
  不适用于: IP

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
  Sequence:

  DYKDDDDK

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: HEK293 cells transfected with vector overexpressing DDDDK tag; ICC/ IF: transfected HEK293 cells.

• 常规说明

  
  

  FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  F-tag-01

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab18230于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for IP.

• 别名

  hide

• DYKDDDDK (also known as the FLAG® tag sequence) antibody

• DYKDDDDK antibody

• DYKDDDDK epitope tag antibody

• DYKDDDDK tag antibody

• ECS epitope tag antibody

• ECS tag antibody

• Enterokinase Cleavage Site epitope tag antibody

• Enterokinase Cleavage Site tag antibody

• FLAG® antibody

• FLAG® tag antibody

• DDDDK epitope tag antibody

• DDDK antibody

• ddk antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)Image courtesy of an anonymous AbReview

  ab18230 staining DDDDK tag in Human HeLa, cervical carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% tween20 and blocked with 0.5% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 24 hours at 4°C. Ab8517 (1/200) was used as the secondary antibody

  See Abreview

• 

  Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)

  (a) Fusion of nuclear protein stained with ab18230 (red)
  (b) Cell nuclei stained with DAPI (blue)
  (c) Merge of (a) and (b) confirming nuclear localisation of the fusion protein.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)Image from Ögmundsdóttir MH et al., PLoS One. 2012;7(5):e36616. Epub 2012 May 4. Fig 1.; doi:10.1371/journal.pone.0036616; May 4, 2012, PLoS ONE 7(5): e36616.

  Immunofluorescence analysis of transfected HEK293 cells, staining DDDDK tag with ab18230 at 1/200 dilution.