Anti-Cyclin B1 antibody [V92.1]

• 产品名称

  Anti-Cyclin B1抗体[V92.1]
  参阅全部 Cyclin B1 一抗

• 描述

  小鼠单克隆抗体[V92.1] to Cyclin B1

• 宿主

  Mouse

• 特异性

  V92.1 can immunoprecipitate active CDK1/cyclin B1 complexes.

• 经测试应用

  适用于: ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length protein (Hamster).

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Protein A/G purified

• 克隆

  单克隆

• 克隆编号

  V92.1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab18221于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

ICC/IF: Use at a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

• 数据库链接

• Entrez Gene: 891 Human

• Omim: 123836 Human

• SwissProt: P14635 Human

• Unigene: 23960 Human

• 别名

• CCNB1_HUMAN antibody

• Cyclin B1 antibody

• G2 mitotic specific cyclin B1 antibody

• G2/mitotic-specific cyclin-B1 antibody

• CCNB 1 antibody

• CCNB antibody

• ccnb1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [V92.1] (ab18221)

ICC/IF image of ab18221 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18221, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.