Anti-FOXK1/MNF抗体
参阅全部 FOXK1/MNF 一抗
兔多克隆抗体to FOXK1/MNF
Rabbit
Replenishment batches of our polyclonal antibody, ab18196 are tested in WB. Previous batches were additionally validated in ICC/IF and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab309510.
适用于: WB, IP, ICC/IFmore details
与反应: Human
预测可用于: Mouse
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab18196于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | 1/1000. |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Entrez Gene: 221937 Human
Entrez Gene: 17425 Mouse
SwissProt: P85037 Human
SwissProt: P42128 Mouse
Unigene: 487393 Human
Unigene: 24214 Mouse
FHX antibody
Fork head antibody
Forkhead box protein K1 antibody
FOXJ2 forkhead factor antibody
FOXK1 antibody
FOXK1_HUMAN antibody
Gm10868 antibody
MNF antibody
Myocyte nuclear factor antibody
A630048H08Rik antibody
AI463295 antibody
ENSMUSG00000075577 antibody
Western blot - Anti-FOXK1/MNF antibody (ab18196)This image is courtesy of an anonymous Abreview
All lanes : Anti-FOXK1/MNF antibody (ab18196) at 1/5000 dilution
Lane 1 : Human H1299 cell lysate
Lane 2 : Mouse embryonic stem cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 100 kDawhy is the actual band size different from the predicted?
Exposure time: 1 minute
Western blot - Anti-FOXK1/MNF antibody (ab18196)
All lanes : Anti-FOXK1/MNF antibody (ab18196) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : Jurkat nuclear extract lysate (ab14844)
Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 7 : SW480 whole cell lysate (ab3957)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 95 kDawhy is the actual band size different from the predicted?
Exposure time: 5 minutes
Immunocytochemistry/ Immunofluorescence - Anti-FOXK1/MNF antibody (ab18196)
ICC/IF image of ab18196 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18196, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunoprecipitation - Anti-FOXK1/MNF antibody (ab18196)
FOXK1/MNF - ChIP Grade was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to FOXK1/MNF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18196.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 96kDa: FOXK1 / MNF.
