Anti-HA tag抗体[HA.C5]
参阅全部 HA tag 一抗
小鼠单克隆抗体[HA.C5] to HA tag
Mouse
适用于: WB, ICC/IFmore details
与反应: Species independent
Synthetic peptide from influenza hemagglutinin epitope:
YPYDVPDYA
conjugated to KLH.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Liquid
Shipped at 4°C. Store at +4°C. Do Not Freeze.
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
Purified from TCS
单克隆
HA.C5
IgG3
Abpromise™承诺保证使用ab18181于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (12) | 1/1000. |
| ICC/IF | (4) | Use at an assay dependent concentration. |
HA epitope tag antibody
HA1 antibody
HA2 antibody
hemagglutinin antibody
Hemagglutinin HA1 chain antibody
Hemagglutinin HA2 chain antibody
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.cn/ps/products/18/ab18181/Images/ab18181-212146-anti-ha-tag-antibody-hac5-western-blot.jpg)
Western blot - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an anonymous Abreview
All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution
Lanes 1-2 : HEK293 whole cell lysate - transfected
Lane 3 : HEK293 whole cell lysate - untransfected
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 85 kDawhy is the actual band size different from the predicted?
Exposure time: 5 minutes
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.cn/ps/products/18/ab18181/Images/ab18181-26826-anti-ha-tag-antibody-hac5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an Abreview submitted by Harendra Chahar
ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.cn/ps/products/18/ab18181/Images/ab18181-26829-anti-ha-tag-antibody-hac5-western-blot.jpg)
Western blot - Anti-HA tag antibody [HA.C5] (ab18181)
Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).
![Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.cn/ps/products/18/ab18181/Images/ab18181-26828-anti-ha-tag-antibody-hac5-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HA tag antibody [HA.C5] (ab18181)
Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).
![Western blot - Anti-HA tag antibody [HA.C5] (ab18181)](https://www.abcam.cn/ps/products/18/ab18181/Images/ab18181-26827-anti-ha-tag-antibody-hac5-western-blot.jpg)
Western blot - Anti-HA tag antibody [HA.C5] (ab18181)This image is courtesy of an anonymous Abreview
All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution
Lane 1 : WCE from cell line transfected for HA-tagged protein
Lane 2 : WCE from a cell line transfected with empty vector
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-mouse IgG (H+L)
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.
