Anti-HEF1/NEDD-9 antibody [2G9]

• 产品名称

  Anti-HEF1/NEDD-9抗体[2G9]
  参阅全部 HEF1/NEDD-9 一抗

• 描述

  小鼠单克隆抗体[2G9] to HEF1/NEDD-9

• 宿主

  Mouse

• 特异性

  Not tested on Sin1. This antibody mostly detects HEF1 / NEDD-9 localized at the focal adhesion sites.

• 经测试应用

  适用于: IP, ICC/IF, IHC-Fr, ICC, IHC-P, WB, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Synthetic peptide corresponding to Human HEF1/NEDD-9 aa 50-400.
  Database link: Q14511

• 阳性对照

• WB: Whole cell lysate prepared from a murine neural stem cell line. ICC/IF: MCF7 cells. Flow Cytometry: A549 cells. IHC-P: Human kidney tissue.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  2G9

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab18056于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4739 Human

• Entrez Gene: 18003 Mouse

• Entrez Gene: 291044 Rat

• Omim: 602265 Human

• SwissProt: Q14511 Human

• SwissProt: O35177 Mouse

• Unigene: 37982 Human

• 别名

• CAS2 antibody

• CasL antibody

• CASL_HUMAN antibody

• CASS2 antibody

• Crk associated substrate related antibody

• Crk associated substrate related protein antibody

• CRK-associated substrate-related protein antibody

• dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)) antibody

• dJ49G10.2 antibody

• dJ761I2.1 (enhancer of filamentation (HEF1)) antibody

• dJ761I2.1 antibody

• Enhancer of filamentation 1 antibody

• Enhancer of filamentation 1 p55 antibody

• HEF 1 antibody

• HEF1 antibody

• NEDD-9 antibody

• Nedd9 antibody

• Neural precursor cell expressed developmentally down-regulated protein 9 antibody

• P105 antibody

• Renal carcinoma antigen NY-REN-12 antibody

• Cas like docking antibody

• Cas scaffolding protein family member 2 antibody

• CAS-L antibody

• 

  Western blot - Anti-HEF1/NEDD-9 antibody [2G9] (ab18056)

  (0.1µg/ml) staining in MCF7 cells lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HEF1/NEDD-9 antibody [2G9] (ab18056)

  Immunofluorescence staining of MDA-MB231 cells with the 2G9 antibody at a 1/200 dilution. Cells were fixed in 3.8% PFA for 10 minutes, and staining was performed for 1 hour at room temperature.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HEF1/NEDD-9 antibody [2G9] (ab18056)

  Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton.

  Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue).

  Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HEF1/NEDD-9 antibody [2G9] (ab18056)

  Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton.

  Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue).

  Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

• 

  Immunocytochemistry - Anti-HEF1/NEDD-9 antibody [2G9] (ab18056)

  ICC/IF image of ab18056 stained MCF7 (human breast adenocarcinoma cell line) cells. The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18056, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
  
  

• 

  Flow Cytometry - Anti-HEF1/NEDD-9 antibody [2G9] (ab18056)

  Overlay histogram showing A549 (human lung carcinoma cell line) cells stained with ab18056 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18056, 1 µg/ 1 x 106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/ 1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEF1/NEDD-9 antibody [2G9] (ab18056)

  IHC image of ab18056 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab18056, 10 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.