Anti-SMC5抗体
参阅全部 SMC5 一抗
兔多克隆抗体to SMC5
Rabbit
适用于: IP, WBmore details
与反应: Human
预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human SMC5 aa 1050 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8IY18
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
Antibody was purified using an epitope specific to SMC5 immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab18038于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/5000 - 1/25000. Detects a band of approximately 145 kDa (predicted molecular weight: 129 kDa). |
Entrez Gene: 23137 Human
Omim: 609386 Human
SwissProt: Q8IY18 Human
Unigene: 534189 Human
Unigene: 601181 Human
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Western blot - Anti-SMC5 antibody (ab18038)
Ab18038 staining Human SMC5 at a concentration of 0.1 µg/ml by Western Blot, and 5 µg/0.5 mg lysate by Immunoprecipitation.
Samples:
A) Nuclear extract (10 and 20 µg) from HeLa cells.
B) Whole cell lysate (500 µg) from HeLa and 293T cells.
Detection: Chemiluminescence with exposure times of less than 2 minutes.
Ab18038 staining Human SMC5 at a concentration of 0.1 µg/ml by Western Blot, and 5 µg/0.5 mg lysate by Immunoprecipitation.
Samples:
A) Nuclear extract (10 and 20 µg) from HeLa cells.
B) Whole cell lysate (500 µg) from HeLa and 293T cells.
Detection: Chemiluminescence with exposure times of less than 2 minutes.
Immunoprecipitation - Anti-SMC5 antibody (ab18038)
Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.
Lane 1: ab18038 used for IP at 6 µg per reaction
Lane 2: Control IgG
For blotting immunoprecipitated SMC5, ab18038 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
