Anti-p53抗体
参阅全部 p53 一抗
兔多克隆抗体to p53
Rabbit
适用于: Flow Cyt (Intra), IHC-P, IP, Flow Cyt, WBmore details
与反应: Human
预测可用于: Chimpanzee, Gorilla
Synthetic peptide within Human p53 aa 50-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P04637-1
WB: HEK-293T whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Human lung cancer tissue. Flow Cyt (Intra): Jurkat whole cell lysate.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab17990于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) | Use 1.5µg for 106 cells. 1.5 μg per 1 million cells in a 150 μl reaction. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody | |
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| Flow Cyt | Use 1.5µg for 106 cells. 1.5 μg per 1 million cells in a 150 μl reaction. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
Entrez Gene: 7157 Human
Omim: 191170 Human
SwissProt: P04637 Human
Unigene: 654481 Human
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Western blot - Anti-p53 antibody (ab17990)
All lanes : Anti-p53 antibody (ab17990) at 0.1 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Predicted band size: 53 kDa
Exposure time: 30 seconds
Immunoprecipitation - Anti-p53 antibody (ab17990)
p53 was immunoprecipitated from 1 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab17990 at 3 µg/ml. Western blot was performed from the immunoprecipitate using ab17990 at 1 µg/ml.
Lane 1: ab17990 IP in HEK-293T whole cell lysate.
Lane 2: Ctrl IgG instead of ab17990.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody (ab17990)
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling p53 with ab17990 at 1/5000 dilution. Detection: DAB.
Flow Cytometry (Intracellular) - Anti-p53 antibody (ab17990)
ab17990 at 1.5ug/ml staining p53 in human Jurkat cell line by Flow Cytometery. Cells were fixed in 1.5% paraformaldehyde, and permeabilized in 90% methanol. A FITC-conjugated goat anti rabbit was used as secondary. The black line indicates control anti KLH IgG and the red line repersents ab17990 staining.
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