Anti-IKK alpha (phospho S176 + S180) antibody

• 产品名称

  Anti-IKK alpha (phospho S176 + S180)抗体
  参阅全部 IKK alpha 一抗

• 描述

  兔多克隆抗体to IKK alpha (phospho S176 + S180)

• 宿主

  Rabbit

• 特异性

  Due to sequence homology between the isoforms, this antibody may cross-react with IKKbeta that contains phosphorylated serine 176 and 180. However, we have no direct experimental evidence for this in our records.

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse

• 免疫原

  Synthetic peptide corresponding to IKK alpha (phospho S176 + S180).

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 0.1% BSA
  
  PBS is Ca2+ and Mg2+ free

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab17943于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1147 Human

• Omim: 600664 Human

• SwissProt: O15111 Human

• SwissProt: Q60680 Mouse

• Unigene: 198998 Human

• Unigene: 3996 Mouse

• 别名

• Conserved helix loop ubiquitous kinase antibody

• Conserved helix-loop-helix ubiquitous kinase antibody

• I Kappa B Kinase 1 antibody

• I Kappa B Kinase Alpha antibody

• I-kappa-B kinase 1 antibody

• I-kappa-B kinase alpha antibody

• IkappaB kinase antibody

• IkB kinase alpha subunit antibody

• IkBKA antibody

• IKK 1 antibody

• IKK A antibody

• IKK a kinase antibody

• IKK-A antibody

• IKK-alpha antibody

• IKK1 antibody

• IKKA antibody

• IKKA_HUMAN antibody

• Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody

• Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody

• Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody

• NFKBIKA antibody

• Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody

• Nuclear factor NF kappa B inhibitor kinase alpha antibody

• Nuclear factor NF-kappa-B inhibitor kinase alpha antibody

• Nuclear factor NFkappaB inhibitor kinase alpha antibody

• Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody

• TCF-16 antibody

• TCF16 antibody

• Transcription factor 16 antibody

• chuk antibody

• CHUK1 antibody

• Conserved Helix Loop Helix Ubiquitous Kinase antibody

• Transcription factor 16 antibody

• 

  Western blot - Anti-IKK alpha (phospho S176 + S180) antibody (ab17943)

  All lanes : Anti-IKK alpha (phospho S176 + S180) antibody (ab17943) at 1/500 dilution
  
  Lane 1 : Jurkat cells stimulated with 80 ng/mL TNF-alpha for 5 minutes at 37C; no peptide
  Lane 2 : Jurkat cells stimulated with 80 ng/mL TNF-alpha for 5 minutes at 37C, non-phosphopeptide corresponding to the immunogen
  Lane 3 : Jurkat cells stimulated with 80 ng/mL TNF-alpha for 5 minutes at 37C, a generic phosphoserine containing peptide
  Lane 4 : Jurkat cells stimulated with 80 ng/mL TNF-alpha for 5 minutes at 37C, with the phosphopeptide immunogen
  Lane 5 : Jurkat cells unstimulated, no peptide
  Lane 6 : Jurkat cells stimulated with 80 ng/mL TNF-alpha for 5 minutes at 37C, no peptide
  
  Secondary
  All lanes : Goat F (ab')2 anti-rabbit IgG HRP conjugate
  
  Predicted band size: 84 kDa
  
  
  
  

  Immunoprecipitates prepared from lysates of Jurkat cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF.

  Membranes were either incubated with peptides (lanes 2, 3, and 4) or without peptides (lanes 1, 5 and 6) then blocked with a 5% BSA-TBST buffer overnight at 4°C and incubated with ab17943 at 1/500 dilution for two hours at room temperature in a 3% BSA-TBST buffer.

  After washing, membranes were incubated with goat F (ab')2 anti-rabbit IgG HRP conjugate, and bands were detected using the Pierce SuperSignal™ method. The phospho-signal is induced by the addition of TNF-alpha.

• 

  Immunocytochemistry - Anti-IKK alpha (phospho S176 + S180) antibody (ab17943)

  Immunofluorescence analysis of 70% confluent log phase 50 ng/mL TNF-Alpha treated paraformaldehyde-fixed HeLa cells permeabilized with 0.25% Triton™ X-100 for 10 minutes, blocked with 5% BSA for 1 hour at room temperature.  Panel a) Green: IKK alpha (phospho S176 + S180) was labelled with ab17943 at 1/250 dilution 0.1% BSA and the secondary antibody was Goat anti-Rabbit IgG (H+L) Superclonal™ Alexa Fluor® 488 conjugate at 1/2000 dilution.The primary was incubated in for 3 hours at room temperature and the secondary: for 45 minutes at room temperature. Panel b) Blue nuclear staining with SlowFade® Gold Antifade Mountant with DAPI. Panel C) Red: F-actin staining with 1/300 dilution Rhodamine Phalloidin. Panel d) Merged. Panel e) No primary antibody control. The images were captured at 60X magnification.