Anti-ERK1 + ERK2抗体
参阅全部 ERK1 + ERK2 一抗
兔多克隆抗体to ERK1 + ERK2
Rabbit
适用于: ICC, IHC-P, WBmore details
与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Human ERK1 + ERK2 aa 317-339 (C terminal).
Sequence:
RIT VEEALAHPYL EQYYDPTDE
Database link: P27361
Please note that this is an intracellular epitope.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
phosphate buffered saline without Mg2+ and
Ca2+.
浓度
50 µl 浓度为 0.3 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab17942于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (1) | Use a concentration of 1 µg/ml. |
| IHC-P | (2) | 1/10 - 1/100. |
| WB | (15) | 1/1000. Predicted molecular weight: 42-44 kDa. |
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SwissProt: P27361 Human
SwissProt: P28482 Human
SwissProt: P63085 Mouse
SwissProt: Q63844 Mouse
Unigene: 431850 Human
Unigene: 196581 Mouse
Unigene: 34914 Rat
Entrez Gene: 5594 Human
Entrez Gene: 5595 Human
Entrez Gene: 26413 Mouse
Entrez Gene: 26417 Mouse
Omim: 176948 Human
Omim: 601795 Human
Mainly expressed in the cytoplasm and only localizes to the nucleus with treatment.
ERK-2 antibody
ERK1 antibody
erk1/2 antibody
ERK2 antibody
ERT1 antibody
ERT2 antibody
Extracellular signal regulated kinase 1 antibody
Extracellular signal-regulated kinase 1 antibody
Extracellular signal-regulated kinase 2 antibody
HS44KDAP antibody
HUMKER1A antibody
Insulin-stimulated MAP2 kinase antibody
MAP kinase 1 antibody
MAP kinase 2 antibody
MAP kinase 3 antibody
MAP kinase isoform p42 antibody
MAP kinase isoform p44 antibody
MAPK 1 antibody
MAPK 2 antibody
MAPK 3 antibody
Mapk1 antibody
MAPK2 antibody
MAPK3 antibody
Microtubule-associated protein 2 kinase antibody
Mitogen-activated protein kinase 1 antibody
Mitogen-activated protein kinase 2 antibody
Mitogen-activated protein kinase 3 antibody
MK01_HUMAN antibody
p38 antibody
p40 antibody
p41 antibody
p41mapk antibody
p42-MAPK antibody
P42MAPK antibody
p44-ERK1 antibody
p44-MAPK antibody
p44ERK1 antibody
p44MAPK antibody
PRKM 2 antibody
PRKM1 antibody
PRKM2 antibody
PRKM3 antibody
protein tyrosine kinase ERK2 antibody
ERK 1 antibody
ERK 2 antibody
ERK-1 antibody
Western blot - Anti-ERK1 + ERK2 antibody (ab17942)
All lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution
Lane 1 : K562 cells
Lane 2 : Daudi cells
Lane 3 : Hep G2 cells
Lane 4 : PC-3 cells
Lane 5 : NIH 3T3 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG - HRP Secondary Antibody at 1/5000 dilution
Predicted band size: 42-44 kDa
Immunocytochemistry - Anti-ERK1 + ERK2 antibody (ab17942)
Immunofluorescent analysis of ERK1 + ERK2 Antibody was done on 70% confluent log phase U87-MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ab17942 at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic and nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)
Immunohistochemistry analysis of ERK1/2 (pan) showing staining in the cytoplasm and nucleus of paraffin-embedded mouse stomach tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab17942 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Western blot - Anti-ERK1 + ERK2 antibody (ab17942)
Western Blot for ab17942.
Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated
with goat anti-rabbit IgG alkaline phosphatase.
These data show that ab17942 ERK1&2 antibody allows the total amount of ERK1&2 to be measured.
Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat anti-ra
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)
Immunohistochemistry analysis of ERK1/2 (pan) showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab17942 diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)
Immunohistochemistry analysis of ERK1/2 (pan) showing staining in the cytoplasm and nucleus of paraffin-embedded human colon carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab17942 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Western blot - Anti-ERK1 + ERK2 antibody (ab17942)This image is courtesy of an anonymous Abreview
All lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution
Lane 1 : Rat spinal cord tissue homogenate from animals that underwent Sham surgery
Lanes 2-3 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit antibody at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42-44 kDa
Observed band size: 42,44 kDawhy is the actual band size different from the predicted?
Exposure time: 5 minutes
The tissue was harvested seven days post surgery, sonicated with RIPA buffer and the protein estimate made by Lowry. A 10% SDS-PAGE gel was run for 1.5 hr at 100V and transfered to PVDF membrane for 1.5 hr at 274 mA. The blot was blocked with 5% BSA for 1 hour at 23°C. The primary antibody was incubated with the blot for 18 hours at 4°C.
Western blot - Anti-ERK1 + ERK2 antibody (ab17942)Image from PLoS One. 2014; 9(6): e99219. Fig3A, doi: 10.1371/journal.pone.0099219 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Western blot analysis of Mice retinas (40-50μg/lane) labelling with anti-ERK1/2 at 1:300 (ab17942) and mouse monoclonal anti-phosphorylated ERK1/2 at 1:300 (ab50011), in 5% nonfat milk in TBST overnight at 4ºC. HRP conjugated antibodies were used as the secondary antibodies.
Data is expressed as percentage change in phosphorylated ERK1/2 (p-ERK1/2) over total ERK1/2 (t-ERK1/2) calculated in control and diabetic mice maintained with and without Edaravone treatment
Results are expressed as mean±SD. Values obtained from Normal group are considered as 100%. *P<0.05, ***P<0.001 vs. Normal, #P<0.05 vs. Edaravone
