Anti-SHP2 (phospho Y542) antibody

• 产品名称

  Anti-SHP2 (phospho Y542)抗体
  参阅全部 SHP2 一抗

• 描述

  兔多克隆抗体to SHP2 (phospho Y542)

• 宿主

  Rabbit

• 经测试应用

  适用于: IHC-P, ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide corresponding to Human SHP2 (phospho Y542). The sequence is conserved in mouse, rat and chicken.

• 阳性对照

• WB: NIH3T3 cells treated with PDGF. IHC-P: Human brain tissue. ICC/IF: A-431

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.

• 存储溶液

  pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated SHP2. The final product is generated by affinity chromatography using a SHP2 derived peptide that is phosphorylated at tyrosine 542.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab17939于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5781 Human

• Entrez Gene: 19247 Mouse

• Omim: 176876 Human

• SwissProt: Q06124 Human

• SwissProt: P35235 Mouse

• Unigene: 506852 Human

• Unigene: 474046 Mouse

• Unigene: 8681 Mouse

• 别名

• METCDS antibody

• MGC14433 antibody

• NS1 antibody

• OTTHUMP00000166107 antibody

• OTTHUMP00000166108 antibody

• Protein tyrosine phosphatase 2 antibody

• Protein tyrosine phosphatase 2C antibody

• Protein tyrosine phosphatase non receptor type 11 antibody

• Protein-tyrosine phosphatase 1D antibody

• Protein-tyrosine phosphatase 2C antibody

• PTN11_HUMAN antibody

• PTP-1D antibody

• PTP-2C antibody

• PTP1D antibody

• PTP2C antibody

• PTPN11 antibody

• SAP2 antibody

• SH-PTP2 antibody

• SH-PTP3 antibody

• SH2 domain containing protein tyrosine phosphatase 2 antibody

• SHP 2 antibody

• SHP-2 antibody

• Shp2 antibody

• SHPTP2 antibody

• SHPTP3 antibody

• Syp antibody

• Tyrosine-protein phosphatase non-receptor type 11 antibody

• BPTP3 antibody

• CFC antibody

• JMML antibody

• 

  Western blot - Anti-SHP2 (phospho Y542) antibody (ab17939)

  Western blot using ab17939 on 10-30µg NIH3T3 cell lysate. Lane 1: untreated cells. Lane 2: cells treated with PDGF. Lane 3: cells treated with PDGF. Antibody blocked with non-phosphorylated immunopeptide. Lane 4: cells treated with PDGF. Antibody blocked with a generic tyrosine-phosphorylated peptide. Lane 5: cells treated with PDGF. Antibody blocked with phosphorylated immunopeptide.

  Western blot using ab17939 on 10-30µg NIH3T3 cell lysate.

  Lane 1: untreated cells.
  Lane 2: cells treated with PDGF.
  Lane 3: cells treated with PDGF. Antibody blocked with non-phosphorylated immunopeptide.
  Lane 4: cells treated with PDGF. Antibody blocked with a generic tyrosine-phosphorylated peptide.
  Lane 5: cells treated with PDGF. Antibody blocked with phosphorylated immunopeptide.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP2 (phospho Y542) antibody (ab17939)

  Immunohistochemical analysis of paraffin-embedded human brain labeling SHP2 (phospho Y542) with ab17939 at 1/20 dilution  (right) compared to a negative control without primary antibody (left).

  To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab17939 diluted in 3% BSA-PBS at a dilution of 1/20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SHP2 (phospho Y542) antibody (ab17939)

  Immunofluorescence analysis of 90% confluent log phase A-431 (Human epidermoid carcinoma cell line) cells treated with 0.2 ug/mL of EGF for 10 minutes labeling SHP2 (phospho Y542)  with ab17939 at 1/250 dilution.

  The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab17939 at 1/250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing membrane localization. Panel e represents cells treated with antagonist, Afatinib (0.5 uM for 6hrs) followed by EGF (0.2 ug/mL for 10 minutes), showing reduced expression of SHP2 (phospho Y542). Panel f shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.