Anti-Milk Fat Globule 1抗体[SPM291]
参阅全部 Milk Fat Globule 1 一抗
小鼠单克隆抗体[SPM291] to Milk Fat Globule 1
Mouse
适用于: IHC-Pmore details
与反应: Human
The details of the immunogen for this antibody are not available.
IHC: Human breast carcinoma, renal cell carcinoma, pancreas and lung carcinoma tissue
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.0268% PBS, BSA
浓度
500 µl 浓度为 0.2 mg/ml
Protein G purified
单克隆
SPM291
IgG1
kappa
Abpromise™承诺保证使用ab17787于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Entrez Gene: 4240 Human
Omim: 602281 Human
SwissProt: Q08431 Human
Unigene: 3745 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
IHC image of ab17787 staining in human carinoma Renal Cell formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
IHC image of ab17787 staining in human carinoma breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Milk Fat Globule 1 with ab18878 at 1 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling Milk Fat Globule 1 with ab18878 at 1 ug/ml.