Anti-PLK1 antibody [35-206]

• 产品名称

  Anti-PLK1抗体[35-206]
  参阅全部 PLK1 一抗

• 描述

  小鼠单克隆抗体[35-206] to PLK1

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt (Intra), ICC, WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Rat

• 免疫原

  Recombinant full length protein corresponding to Human PLK1.

• 表位

  aa330-370.

• 阳性对照

• WB: A431, U-2 OS and HeLa S3 lysates. Flow Cyt (Intra): HCT 116 cells. ICC/IF: HeLa cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  35-206

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab17056于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5347 Human

• Entrez Gene: 25515 Rat

• Omim: 602098 Human

• SwissProt: P53350 Human

• SwissProt: Q62673 Rat

• Unigene: 592049 Human

• Unigene: 11034 Rat

• 别名

• PLK-1 antibody

• plk1 antibody

• PLK1_HUMAN antibody

• Polo like kinase 1 antibody

• Polo-like kinase 1 antibody

• Serine/threonine protein kinase 13 antibody

• Serine/threonine protein kinase PLK1 antibody

• Serine/threonine-protein kinase 13 antibody

• Serine/threonine-protein kinase PLK1 antibody

• STPK 13 antibody

• STPK13 antibody

• Cell cycle regulated protein kinase antibody

• PLK 1 antibody

• PLK antibody

• 

  Immunocytochemistry - Anti-PLK1 antibody [35-206] (ab17056)

  ab264082 staining PLK1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab264082 at 1μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• 

  Western blot - Anti-PLK1 antibody [35-206] (ab17056)

  Anti-PLK1 antibody [35-206] (ab17056) at 1 µg/ml + A431 cell lysate at 20 µg
  
  Secondary
  Rabbit-anti mouse Alexa fluor(680) at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 67 kDa
  Observed band size: 66 kDawhy is the actual band size different from the predicted?
  
  

• 

  Western blot - Anti-PLK1 antibody [35-206] (ab17056)

  All lanes : Anti-PLK1 antibody [35-206] (ab17056)
  
  Lane 1 : Recombinant PLK1
  Lane 2 : U2OS lysate
  Lane 3 : HeLaS3
  
  Performed under reducing conditions.
  
  Predicted band size: 67 kDa
  Observed band size: 66 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Western blot using ab17056.
  
  Lane 1: recombinant PLK1
  Lane 2: U2OS lysate
  Lane 3: HeLaS3 lysate
  
  15% SDS-PAGE gel

• 

  Immunocytochemistry - Anti-PLK1 antibody [35-206] (ab17056)This image is courtesy of an Abreview submitted by Melanie Adler

  ab17056 at 1/100 staining human kidney tubular epithelial cells by ICC/IF. The cells were formaldehyde fixed, permeabilized with Triton X-100 and blocked with goat serum before incuabtion with the antibody. A goat anti-mouse FITC antibody was used as the secondary.

  See Abreview

• 

  Flow Cytometry (Intracellular) - Anti-PLK1 antibody [35-206] (ab17056)

  Overlay histogram showing HCT116 cells stained with ab17056 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17056, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.