Anti-Caveolin-1 antibody [7C8]

• 产品名称

  Anti-Caveolin-1抗体[7C8]
  参阅全部 Caveolin-1 一抗

• 描述

  小鼠单克隆抗体[7C8] to Caveolin-1

• 宿主

  Mouse

• 特异性

  The monoclonal antibody 7C8 recognizes caveolin-1a as well as caveolin-1ß, which are present in many tissues, like aorta, heart, muscle, lung, adipose white, brown and epidydimal fat.

• 经测试应用

  适用于: ICC/IF, WBmore details
  不适用于: Flow Cyt

• 种属反应性

  与反应: Rat, Human
  预测可用于: Mouse

• 免疫原

  Tissue, cells or virus corresponding to Rat Caveolin-1. GLUT4-containing vesicles immunoadsorbed from low density microsomes of rat adipocytes (Sprague Dawley). The antibody recognises epitope between residue 32 and the C-terminus.

• 阳性对照

• WB: A431, A549 and HUVEC cell lysates. ICC: HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituents: PBS, 0.1% BSA

• 纯度

  Protein G purified

• 纯化说明

  0.2 µm filtered

• 克隆

  单克隆

• 克隆编号

  7C8

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab17052于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for Flow Cyt.

• 数据库链接

  hide

• Unigene: 28278 Mouse

• Unigene: 470537 Mouse

• Unigene: 22518 Rat

• Entrez Gene: 857 Human

• Entrez Gene: 12389 Mouse

• Entrez Gene: 25404 Rat

• Omim: 601047 Human

• SwissProt: Q03135 Human

• SwissProt: P49817 Mouse

• SwissProt: P41350 Rat

• Unigene: 74034 Human

• 别名

• CAV1_HUMAN antibody

• caveolae protein, 22 kD antibody

• caveolin 1 alpha isoform antibody

• caveolin 1 beta isoform antibody

• Caveolin 1 caveolae protein 22kDa antibody

• Caveolin-1 antibody

• Caveolin1 antibody

• cell growth-inhibiting protein 32 antibody

• CGL3 antibody

• LCCNS antibody

• MSTP085 antibody

• OTTHUMP00000025031 antibody

• PPH3 antibody

• VIP 21 antibody

• VIP21 antibody

• BSCL3 antibody

• CAV antibody

• CAV1 antibody

• 

  Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)

  All lanes : Anti-Caveolin-1 antibody [7C8] (ab17052) at 1 µg/ml
  
  Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
  Lane 2 : CAV1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
  Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
  Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Performed under reducing conditions.
  
  Observed band size: 21-24 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Lanes 1 - 4: Merged signal (red and green). Green - ab17052 observed at 21-24 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

  ab17052 was shown to react with Caveolin-1 in wild-type A431 cells in western blot. Loss of signal was observed when CAV1 knockout sample was used. Wild-type and CAV1 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in non-mammalian (TBS-based) blocking solution before incubation with ab17052 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [7C8] (ab17052)

  ab17052 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab17052 at 1/500 dilution and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
  Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous abreview.

   ab17052 staining Caveolin-1 in human Hacat keratinocyte cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 1% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/75 for 24 hours at 4°C. The secondary antibody used was conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

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• 

  Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous Abreview.

  Anti-Caveolin-1 antibody [7C8] (ab17052) at 1/500 dilution + HUVEC whole cell lysate at 20 µg
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Exposure time: 10 seconds
  

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• 

  Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous Abreview.

  Immunocytochemical analysis of Mouse Bend.3 cells, labeling Caveolin-1 with ab17052. Cells were paraformaldehyde fixed, permeabilized with PBS + 0.1% Triton (PBT), and blocked with 10% serum for 1 hour at 22°C. Staining with ab17052 (diluted 1/200) was for 16 hours at 4°C.

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• 

  Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous Abreview

  ab17052 at a 1/500 dilution staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody. Bound antibody was detected using a FITC conjugated goat anti-mouse antibody.

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