Anti-FOXO3A antibody

• 产品名称

  Anti-FOXO3A抗体
  参阅全部 FOXO3A 一抗

• 描述

  山羊多克隆抗体to FOXO3A

• 宿主

  Goat

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide:

  GAKQASSQSWVPG

  , corresponding to amino acids 661-673 of Human FOXO3A.

  Run BLAST with Run BLAST with 

• 阳性对照

• Recombinant Human FOXO3A protein (ab114191) can be used as a positive control in WB. Human heart tissue lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: Tris buffered saline, 0.5% BSA

• 浓度

• 100 µg 浓度为 0.5 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab17026于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2309 Human

• Omim: 602681 Human

• SwissProt: O43524 Human

• Unigene: 220950 Human

• 别名

• FKHR2 antibody

• FKHRL 1 antibody

• FKHRL1 antibody

• FKHRL1P2 antibody

• Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody

• Forkhead box O3 antibody

• Forkhead box O3A antibody

• Forkhead box protein O3 antibody

• Forkhead box protein O3A antibody

• Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody

• Forkhead homolog (rhabdomyosarcoma) like 1 antibody

• Forkhead in rhabdomyosarcoma like 1 antibody

• Forkhead in rhabdomyosarcoma-like 1 antibody

• FOX O3A antibody

• FOXO2 antibody

• foxo3 antibody

• FOXO3_HUMAN antibody

• FOXO3A antibody

• MGC12739 antibody

• MGC31925 antibody

• AF6q21 antibody

• AF6q21 protein antibody

• DKFZp781A0677 antibody

• 

  Western blot - Anti-FOXO3A antibody (ab17026)

  All lanes : Anti-FOXO3A antibody (ab17026) at 0.3 µg/ml
  
  Lane 1 : Human Heart tissue lysate
  Lane 2 : Human Heart tissue lysate with immunising peptide
  
  Lysates/proteins at 35 µg per lane.
  
  Predicted band size: 72 kDa
  Observed band size: 70 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Western blot - Anti-FOXO3A antibody (ab17026)This image is courtesy of an anonymous Abreview

  All lanes : Anti-FOXO3A antibody (ab17026) at 1/1000 dilution
  
  All lanes : Human hepatic epithelial-like cell line (AKN-1) whole cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Alkaline Phosphatase conjugated-mouse anti-goat IgG monoclonal at 1/15000 dilution
  
  Developed using the ECL technique.
  
  Performed under non-reducing conditions.
  
  Predicted band size: 72 kDa
  Observed band size: 95 kDawhy is the actual band size different from the predicted?
  Additional bands at: 100 kDa (possible non-specific binding)
  
  
  Exposure time: 5 minutes
  
  
  

  Blocked for 1 hour at 22°C.

  Incubated with the primary antibody for 20 hours at 4°C.

  See Abreview