Anti-Ras antibody [F132-62]

• 产品名称

  Anti-Ras抗体[F132-62]
  参阅全部 Ras 一抗

• 描述

  小鼠单克隆抗体[F132-62] to Ras

• 宿主

  Mouse

• 特异性

  Reactive with the Ras translational products of the H-, K- and N-ras human, as well as mouse and rat c- and v-ras oncogenes. We have received mixed results for Western Blot.

• 经测试应用

  适用于: Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein (Human).

• 阳性对照

• Sw480 or Y1 cells or normal skin tissue.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Store at +4°C short term (1-2 weeks). Store at +4°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituents: 0.2% Gelatin, 0.82% Sodium phosphate

• 纯度

  Tissue culture supernatant

• 纯化说明

  Purified from TCS.

• 克隆

  单克隆

• 克隆编号

  F132-62

• 骨髓瘤

  P3x63-Ag8.653

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab16907于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• SwissProt: P01116 Human

• Unigene: 37003 Human

• Entrez Gene: 3265 Human

• Entrez Gene: 3845 Human

• Entrez Gene: 4893 Human

• Omim: 164790 Human

• Omim: 190020 Human

• Omim: 190070 Human

• SwissProt: P01111 Human

• SwissProt: P01112 Human

• 别名

• c-H-ras antibody

• C-HA-RAS1 antibody

• CTLO antibody

• GTPase HRas antibody

• GTPase KRas antibody

• GTPase NRas antibody

• H ras antibody

• H RASIDX antibody

• H-Ras-1 antibody

• H-RASIDX antibody

• Ha-Ras antibody

• HAMSV antibody

• HRAS antibody

• HRAS1 antibody

• K ras antibody

• K RAS2A antibody

• K RAS2B antibody

• K RAS4A antibody

• K RAS4B antibody

• K-RAS antibody

• KRAS antibody

• KRAS1 antibody

• KRAS2 antibody

• N-RAS antibody

• N-terminally processed antibody

• NRAS antibody

• NRAS1 antibody

• p21ras antibody

• RASH_HUMAN antibody

• RASH1 antibody

• RASK2 antibody

• Transforming protein p21 antibody

• v Ha ras Harvey rat sarcoma viral oncogene homolog antibody

• v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody

• v ras neuroblastoma RAS viral oncogene homolog antibody

• C BAS/HAS antibody

• C HA RAS1 antibody

• C-BAS/HAS antibody

• 

  Flow Cytometry - Anti-Ras antibody [F132-62] (ab16907)

  Overlay histogram showing HeLa cells stained with ab16907 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16907, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  This image was generated using the ascites version of the product.