Anti-Calretinin antibody [SP13]

• 产品名称

  Anti-Calretinin抗体[SP13]
  参阅全部 Calretinin 一抗

• 描述

  兔单克隆抗体[SP13] to Calretinin

• 宿主

  Rabbit

• 经测试应用

  适用于: IHC-P, IHC-Frmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Recombinant full length protein within Mouse Calretinin. The exact sequence is proprietary.

• 阳性对照

• IHC-P: Human mesothelioma. IHC-Fr: Mouse and rat cerebrum tissue

• 常规说明

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

  
  

  This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.20
  Preservative: 0.1% Sodium azide
  Constituents: 1% BSA, 98.9% PBS

• 浓度

• 1 ml 浓度为 0.037 mg/ml

• 500 µl 浓度为 0.037 mg/ml

• 100 µl 浓度为 0.037 mg/ml

• 500 µl 浓度为 0.037 mg/ml

• 100 µl 浓度为 0.037 mg/ml

• 40 µl 浓度为 0.037 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  SP13

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab16694于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

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• Unigene: 2755 Mouse

• Unigene: 10321 Rat

• Entrez Gene: 794 Human

• Entrez Gene: 12308 Mouse

• Entrez Gene: 117059 Rat

• Omim: 114051 Human

• SwissProt: P22676 Human

• SwissProt: Q08331 Mouse

• SwissProt: P47728 Rat

• Unigene: 106857 Human

• 别名

• CAL 2 antibody

• CAL2 antibody

• CALB 2 antibody

• CALB2 antibody

• CALB2_HUMAN antibody

• Calbindin 2 29kDa antibody

• Calbindin 2 antibody

• Calbindin D29K antibody

• Calbindin2 antibody

• Calretinin antibody

• CR antibody

• 29 kDa calbindin antibody

• CAB 29 antibody

• CAB29 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [SP13] (ab16694)

  Immunohistochemical analysis of paraffin-embedded Human mesothelioma labeling Calretinin with ab16694 at 1/100 (27 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16694 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.

• 

  Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

  This image was generated using a previous batch manufactured using hybridoma production method.

  Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• 

  Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

  This image was generated using a previous batch manufactured using hybridoma production method.

  Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• 

  Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

  This image was generated using a previous batch manufactured using hybridoma production method.

  Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• 

  Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

  This image was generated using a previous batch manufactured using hybridoma production method.

  Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.