Anti-Progesterone Receptor antibody [SP2]

• 产品名称

  Anti-Progesterone Receptor抗体[SP2]
  参阅全部 Progesterone Receptor 一抗

• 描述

  兔单克隆抗体[SP2] to Progesterone Receptor

• 宿主

  Rabbit

• 经测试应用

  适用于: ICC/IF, IHC-P, mIHC, Flow Cytmore details

• 种属反应性

  与反应: Human
  预测可用于: Rat, Rabbit

• 免疫原

  Recombinant fragment. This information is considered to be commercially sensitive.

• 阳性对照

• Breast carcinomas IHC-P: Human breast carcinoma tissue. ICC/IF: T-47D cells Flow Cyt: T-47D cells mIHC: Human mammary gland tissue sections, Human triple-positive breast carcinoma tissue sections

• 常规说明

  This product was switched from a hybridoma to recombinant production method on 13th November 2019.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

  Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

  
  

  This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

• 存储溶液

  pH: 7.20
  Preservative: 0.1% Sodium azide
  Constituents: 1% BSA, PBS

• 浓度

• 批次浓度范围 100 µl 浓度为 0.075 - 0.08 mg/ml

• 批次浓度范围 1 ml 浓度为 0.075 - 0.085 mg/ml

• 500 µl 浓度为 0.075 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  SP2

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab16661于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

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• Unigene: 10303 Rat

• Entrez Gene: 5241 Human

• Entrez Gene: 100009094 Rabbit

• Entrez Gene: 25154 Rat

• Omim: 607311 Human

• SwissProt: P06401 Human

• SwissProt: Q63449 Rat

• Unigene: 32405 Human

• Unigene: 742403 Human

• 别名

• PR antibody

• PRA antibody

• PRB antibody

• PRGR_HUMAN antibody

• Progesterone receptor antibody

• Progestin receptor form A antibody

• Progestin receptor form B antibody

• NR3C3 antibody

• Nuclear receptor subfamily 3 group C member 3 antibody

• PGR antibody

• 

  Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

  Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-positive breast carcinoma tissue sections labeling Progesterone Receptor (PR) with ab16661, at a 1/6000 dilution ( 0.2 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.

  Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-positive breast carcinoma.
  Panel B: anti-PR stained on nucleus of cancer cells.
  Panel C: anti-HER2 stained on membrane of cancer cells.
  Panel D: anti-ER stained on nucleus of cancer cells.

  The section was incubated in three rounds of staining: in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)

  Immunocytochemistry/ Immunofluorescence analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/100 (2.28 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  This image was generated from the hybridoma version.

   

• 

  Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

  Flow Cytometry analysis of T-47D (human ductal breast epithelial tumor epithelial cell) cells labeling Progesterone Receptor with purified ab16661 at 1/220 dilution (1.04 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

  This image was generated from the hybridoma version.

   

• 

  Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

  Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mammary gland tissue sections labeling Progesterone Receptor (PR) with ab16661, at a 1/6000 dilution ( 0.2 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.

  Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human mammary gland.
  Panel B: anti-PR stained on nucleus of some ductal cells.
  Panel C: anti-HER2 stained on no cells.
  Panel D: anti-ER stained on nucleus of some ductal cells.

  The section was incubated in three rounds of staining: in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

  Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human triple-negative breast carcinoma tissue sections labeling Progesterone Receptor (PR) with ab16661, at a 1/6000 dilution ( 0.2 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins and Opal Polymer HRP Ms + Rb was used as the secondary antibody. DAPI was used as the nuclear counterstain.

  Panel A: merged staining of anti-Progesterone Receptor (PR) (magenta; Opal™690), anti-HER2 (red; Opal™570) and anti-Estrogen Receptor (ER) (green; Opal™520) on human triple-negative breast carcinoma.
  Panel B: anti-PR stained on no cells.
  Panel C: anti-HER2 stained on no cells.
  Panel D: anti-ER stained on no cells.

  The section was incubated in three rounds of staining: in the order of ab16661 for 30 mins, then ab16660 and ab231438 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [SP2] (ab16661)

  Immunohistochemistry analysis of human breast carcinoma tissue labelling SP2 with ab16661.

  This image was generated from the hybridoma version.

   

• 

  Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (ab16661)

  Overlay histogram showing T47D cells stained with ab16661 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16661, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  This image was generated from the hybridoma version.

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor antibody [SP2] (ab16661)Ding L, et al. (2019), PLOS Genetics, 15(3), e1008002. Fig 3D, DOI: 10.1371/journal.pgen.1008002. Reproduced under the Creative Commons licence. https://creativecommons.org/licenses/by/4.0/

  Rat mammary epithelial cells stained for Pr (pink) using ab16661 at 1/100 dilution in ICC/IF. 

• 

  Anti-Progesterone Receptor antibody [SP2]