Anti-pan Cadherin抗体
参阅全部 pan Cadherin 一抗
兔多克隆抗体to pan Cadherin
Rabbit
适用于: ICC/IF, WB, IHC-Pmore details
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Xenopus laevis, Zebrafish
Synthetic peptide corresponding to Human pan Cadherin aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab17098)
ICC/IF: U2OS; HeLa cells. WB: Mouse Heart; Mouse Muscle; Human Heart; Rat Heart. IHC-P: Human Liver.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.3 - 0.7 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab16505于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (4) | Use a concentration of 2 µg/ml. A diffuse signal is seen throughout the cells if higher concentrations are used (5-10µg/ml). We have had reports that the antibody works less well in this application in murine (3T3) cells. |
| WB | (2) | Use a concentration of 1 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 100 kDa). |
| IHC-P | (2) | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Entrez Gene: 1012 Human
Entrez Gene: 1014 Human
Entrez Gene: 1015 Human
Entrez Gene: 999 Human
Omim: 114020 Human
Omim: 114021 Human
Omim: 192090 Human
Omim: 601120 Human
Omim: 603006 Human
SwissProt: P12830 Human
SwissProt: P19022 Human
SwissProt: P22223 Human
SwissProt: P33151 Human
SwissProt: P55283 Human
SwissProt: P39038 Mouse
Entrez Gene: 1001 Human
Entrez Gene: 1002 Human
Entrez Gene: 1003 Human
Entrez Gene: 1004 Human
Entrez Gene: 1005 Human
Entrez Gene: 1006 Human
Entrez Gene: 1008 Human
Entrez Gene: 1009 Human
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Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody (ab16505)
ab16505 staining pan Cadherin in U2OS cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16505 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Western blot - Anti-pan Cadherin antibody (ab16505)
All lanes : Anti-pan Cadherin antibody (ab16505) at 1 µg/ml
Lane 1 : Human heart lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa
Observed band size: 125-140 kDawhy is the actual band size different from the predicted?
Additional bands at: 40 kDa, 65 kDa, 75 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cadherin antibody (ab16505)
IHC image of pan Cadherin staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16505, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Anti-pan Cadherin antibody (ab16505)
All lanes : Anti-pan Cadherin antibody (ab16505) at 1 µg/ml
Lane 1 : Mouse heart
Lane 2 : HeLa cell lysate
Lane 3 : 3T3 cell lysate
Lane 4 : Mouse muscle
Lane 5 : Human heart
Lane 6 : Mouse heart with Human pan Cadherin peptide (ab17098) at 1 µg/ml
Lane 7 : HeLa cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/ml
Lane 8 : 3T3 cell lysate with Human pan Cadherin peptide (ab17098) at 1 µg/ml
Lane 9 : Mouse muscle with Human pan Cadherin peptide (ab17098) at 1 µg/ml
Lane 10 : Human heart with Human pan Cadherin peptide (ab17098) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit conjugated to Alexafluor 680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 100 kDa
Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody (ab16505)This image is courtesy of Rosmaria Mangiacasale & Patrizia Lavia, University La Sapienza
HeLa cells fixed in methanol and stained with ab16505 (2µg/ml). The cells were fixed in 100% methanol for 6 minutes at -20°C, then washed once in PBS. The 2 images show the cells stained with different secondary antibodies, Donkey anti Rabbit FITC (image A) and Donkey anti Rabbit Cy3 (image B). In each case ab16505 stains the plasma membrane. In image A ab16505 is stained green and in image B ab16505 is stained red. In both images the DNA is stained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-pan Cadherin antibody (ab16505)Image from Kiss K et al., PLoS One. 2012;7(5):e37378. Epub 2012 May 24. Fig 1.; doi:10.1371/journal.pone.0037378; May 24, 2012, PLoS ONE 7(5): e37378.
Immunofluorescence analysis of HeLa cells, staining pan Cadherin (red) with ab16505.
Cells were fixed with paraformaldehyde, permeabilized in methanol and blocked for 1 hour at room temperature in DPBS containing 2 mg/mL BSA, 1% fish gelatin, 0.1% Triton-X 100 and 5% goat serum. Cells were then incubated for 1 hour at room temperature with the primary antibody diluted in blocking buffer. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
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