Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker

• 产品名称

  Anti-TRA-1-81抗体[TRA-1-81] - Stem Cell Marker

• 描述

  小鼠单克隆抗体[TRA-1-81] to TRA-1-81 - Stem Cell Marker

• 宿主

  Mouse

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Rabbit不与反应: Mouse

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: HUES7 whole cell lysate.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 纯度

  Tissue culture supernatant

• 纯化说明

  Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS

• 克隆

  单克隆

• 克隆编号

  TRA-1-81

• 骨髓瘤

  P3x66Ag.8-Sp2/0

• 同种型

  IgM

The Abpromise guarantee

Abpromise™承诺保证使用ab16289于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5420 Human

• Omim: 602632 Human

• SwissProt: O00592 Human

• 别名

• Gp200 antibody

• PC antibody

• PCLP antibody

• PCLP1 antibody

• 

  Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)

  Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289) at 3 µg/ml + HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 200-400 kDa
  
  
  Exposure time: 20 minutes
  
  
  

  This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16289 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

• 

  Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)

  Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg
  
  Secondary
  Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 200-400 kDa
  
  
  Exposure time: 3 minutes