Anti-TRA-1-60 (R)抗体[TRA-1-60]
小鼠单克隆抗体[TRA-1-60] to TRA-1-60 (R)
Mouse
适用于: ICC, WBmore details
与反应: Human
预测可用于: Rabbit
Tissue, cells or virus corresponding to Human TRA-1-60 (R). Human embryonal carcinoma cell line 2102Ep cl.2A6.
WB: Human embryonic stem cell lysate. ICC: Human embryonic stem cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Tissue culture supernatant
Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
单克隆
TRA-1-60
P3x66Ag.8-Sp2/0
IgM
Abpromise™承诺保证使用ab16288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/500. | |
| WB | (1) | Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa). |
TRA 1 60 antibody
![Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.cn/ps/products/16/ab16288/Images/ab16288-24939-anti-tra-1-60-r-antibody-tra-1-60-western-blot.jpg)
Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
Predicted band size: 235 kDa
Observed band size: 250 kDawhy is the actual band size different from the predicted?
As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.
![Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.cn/ps/products/16/ab16288/Images/ab16288-24937-anti-tra-1-60-r-antibody-tra-1-60-immunofluorescence.jpg)
Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an Abreview submitted by Ms Fiona Lewis
Immunocytochemical analysis of ab16288 staining TRA-160 (R) in HUES7 cells. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody, dilution 1:500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated goat polyclonal to mouse IgM, dilution 1:100, was used as secondary antibody.
![Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.cn/ps/products/16/ab16288/Images/ab16288-470577-anti-tra-1-60-r-antibody-tra-1-60-immunocytochemistry.jpg)
Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an anonymous collaborator.
This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.
![Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.cn/ps/products/16/ab16288/Images/ab16288-272266-anti-tra-1-60-r-antibody-tra-1-60-immunofluorescence.jpg)
Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an abreview submitted by Vladimir Milenkovic, University Hospital Regensburg, Germany
Immunocytochemistry analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1:500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1:500 dilution.
