Anti-SSEA3抗体[MC631]
大鼠单克隆抗体[MC631] to SSEA3
Rat
适用于: IHC-FoFr, Flow Cyt, ICC/IFmore details
与反应: Human
预测可用于: Mouse
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
2102Ep Human Embryonic Carcinoma Cells
The monoclonal antibody that defines SSEA-3 was derived by fusing cells of the murine myeloma cell line SP2/0 with the spleen cells of a Fisher rat immunized with 4-8-cell-stage zonapellucida-free embryos from randomly bred mice.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Tissue culture supernatant
Tissue culture supernatant was cross flow concentrated.
单克隆
MC631
P3x63-Ag8.653
IgM
Abpromise™承诺保证使用ab16286于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. | |
| Flow Cyt | (1) | 1/50. ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody. |
| ICC/IF | (1) | Use at an assay dependent concentration. |
Stage specific embryonic antigen 3 antibody
![Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)](https://www.abcam.cn/ps/products/16/ab16286/Images/ab16286-316783-anti-ssea3-antibody-mc631-flow-cytometry.jpg)
Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)This image is courtesy of Prof Chris Denning's lab, University of Nottingham
Human induced pluripotent stem cells (iPSCs) stained with ab16286 (red fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16286, 1/50 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 647 goat anti-rat IgG at 1/2000 dilution for 30 min at 4°C. iPSCs differentiated for 28 days toward a cardiomyocyte lineage (blue fill) were used as a negative control. Acquisition of >30,000 total events were collected using a 100mW solid state diode laser (640nm) and 670/30 bandpass filter.
![Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286)](https://www.abcam.cn/ps/products/16/ab16286/Images/ab16286-24923-anti-ssea3-antibody-mc631-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286)This image is courtesy of the laboratory of Peter Andrews.
The image shows cell membrane staining of 2102Ep human embryonic carcinoma cells using SSEA3 specific antibody ab16286.
![Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)](https://www.abcam.cn/ps/products/16/ab16286/Images/ab16286-24924-anti-ssea3-antibody-mc631-flow-cytometry.jpg)
Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)This image is from an anonymous collaborator.
Anti-SSEA3 antibody, ab16286 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. The top histogram (A) shows undifferentiated NTERA2 cells (i.e. without retinoic acid) recognised by ab16286. However, upon differentiation (by the addition of retinoic acid 10-5M for 7 days), the antibody lost the ability to recognise these cells as shown in the bottom histogram (B).
