Anti-COX IV抗体- Mitochondrial Loading Control
参阅全部 COX IV 一抗
兔多克隆抗体to COX IV - Mitochondrial Loading Control
Rabbit
适用于: IHC-Fr, WB, ICC/IF, IHC-Pmore details
与反应: Mouse, Rat, Human
预测可用于: Xenopus laevis, Chimpanzee, Monkey, Potato, African green monkey, Chinese hamster
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ICC/IF: HeLa cells.
This antibody makes an effective loading control for mitochondria. COX IV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COX IV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Mouse monoclonal to COX IV antibody [20E8] (ab14744).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.6 - 1 mg/ml
批次浓度范围 250 µg 浓度为 0.6 - 1 mg/ml
Immunogen affinity purified
This antibody makes an effective loading control for mitochondria. COX IV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COX IV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Mouse monoclonal to COX IV antibody [20E8] (ab14744).
多克隆
IgG
Abpromise™承诺保证使用ab16056于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (2) | Use at an assay dependent concentration. |
| WB | (15) | Use a concentration of 0.5 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa). |
| ICC/IF | (6) | Use a concentration of 1 µg/ml. |
| IHC-P | (5) | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Unigene: 433419 Human
Unigene: 386758 Mouse
Unigene: 2528 Rat
Entrez Gene: 454391 Chimpanzee
Entrez Gene: 1327 Human
Entrez Gene: 12857 Mouse
Omim: 123864 Human
SwissProt: O46577 Chimpanzee
SwissProt: P13073 Human
SwissProt: P19783 Mouse
COX IV antibody
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COX41_HUMAN antibody
Cox4a antibody
COX4B antibody
COX4I1 antibody
COX4I2 antibody
COX4L2 antibody
COXIV antibody
Cytochrome c oxidase polypeptide IV antibody
Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
Cytochrome C Oxidase subunit IV antibody
Cytochrome c oxidase subunit IV isoform 1 antibody
Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
Cytochrome c oxydase subunit 4 antibody
dJ857M17.2 antibody
MGC105470 antibody
MGC72016 antibody
AL024441 antibody
COX 4 antibody
COX IV 1 antibody
Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)This image is courtesy of an anonymous Abreview
All lanes : Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1/3000 dilution
All lanes : mouse heart whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG, HRP-linked Antibody at 1/1000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 17 kDa
Observed band size: 15 kDawhy is the actual band size different from the predicted?
Exposure time: 30 seconds
15% gel
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)This image is courtesy of an anonymous Abreview
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed mouse heart tissue. Stained with ab16056 at 1/500 dilution.
Secondary antibody used was EnVision+ System- HRP Labelled Polymer anti rabbit. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% normal goat serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Citrate pH 6.0
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)This image is courtesy of an anonymous Abreview
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human brain tissue. Stained with ab16056 at 1/150 dilution. Secondary antibody used was EnVision+ System- HRP Labelled Polymer anti rabbit. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% normal goat serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Citrate pH 6.0
Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
All lanes : Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Rat skeletal muscle tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Blocking buffer: 2% BSA
Gel type: MES
Exposure Time: 1 minute
Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
ab16056 staining COX IV in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16056 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
All lanes : Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Blocking buffer: 2% BSA
Gel type: MES
Exposure Time: 1 minute
Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
All lanes : Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)This image is courtesy of an anonymous Abreview
ab16056 staining COX IV in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/600 ) for 12 hours at 4°C. A Cy5® donkey anti-rabbit secondary antibody was used as the secondary antibody.
Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
All lanes : Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 0.38 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : Human skeletal muscle cell lysate
Lane 3 : HeLa whole cell lysate with Human COX IV peptide (ab16381) at 1 µg/ml
Lane 4 : Human skeletal muscle cell lysate with Human COX IV peptide (ab16381) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 15 kDawhy is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
IHC image of COXIV staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16056, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)This image is courtesy of an anonymous Abreview.
ab16056 staining COX IV in breast tumour tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% buffered formalin and blocked with 5% serum for 60 minutes at 21°C; antigen retrieval was by heat mediation in a 10mM sodium citrate buffer pH6. Samples were incubated with primary antibody (1/300 in blocking buffer) for 12 hours at 4°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
