Anti-PAX3抗体
参阅全部 PAX3 一抗
山羊多克隆抗体to PAX3
Goat
适用于: WB, IHC-Pmore details
与反应: Human
预测可用于: Rat, Chicken, Dog
Synthetic peptide corresponding to Human PAX3 aa 1-100 (N terminal).
Database link: P23760-1
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab15717于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 53 kDa. 1 hour primary incubation is recommended for this product. | |
| IHC-P | (2) | Use a concentration of 3 µg/ml. |
Entrez Gene: 5077 Human
Omim: 606597 Human
SwissProt: P23760 Human
Unigene: 42146 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody (ab15717)Image courtesy of an anonymous Abreview.
ab15717 staining PAX3 in murine striated muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrated buffer pH 6. Samples were then blocked with 1% BSA for 30 minutes at 25°C and then incubated with ab15717 at a 1/100 dilution for 18 hours at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody (ab15717)
ab15717 (3µg/ml) staining of paraffin embedded Human Oesophagus. The tissue sections subjected to antigen retrieval by microwave in Tris/EDTA buffer pH 9.0. The HRP-staining procedure was used for detection.
Western blot - Anti-PAX3 antibody (ab15717)
Anti-PAX3 antibody (ab15717) at 0.3 µg/ml + Human duodenum lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 45 kDawhy is the actual band size different from the predicted?
