Anti-SMUG1 antibody

• 产品名称

  Anti-SMUG1抗体
  参阅全部 SMUG1 一抗

• 描述

  山羊多克隆抗体to SMUG1

• 宿主

  Goat

• 经测试应用

  适用于: ICC, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide: PQAFLLGSIHEPA, corresponding to N terminal amino acids 2-14 of SMUG1.

  Run BLAST with  Run BLAST with 

• 阳性对照

• ICC: U2OS and MCF7 cells; Flow Cyt (intra): MCF7 cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: Tris buffered saline, 0.5% BSA

• 浓度

• 100 µg 浓度为 0.5 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab15716于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 23583 Human

• Omim: 607753 Human

• SwissProt: Q53HV7 Human

• Unigene: 632721 Human

• Unigene: 731659 Human

• 别名

• Single strand selective monofunctional uracil DNA glycosylase 1 antibody

• Single strand selective monofunctional uracil DNA glycosylase antibody

• Single-strand selective monofunctional uracil DNA glycosylase antibody

• SMUG 1 antibody

• Smug1 antibody

• SMUG1 protein antibody

• SMUG1_HUMAN antibody

• UNG 3 antibody

• UNG3 antibody

• FDG antibody

• HMUDG antibody

• MGC104370 antibody

• 

  Flow Cytometry (Intracellular) - Anti-SMUG1 antibody (ab15716)

  Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) labelling SMUG1 with ab15716. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.

   

• 

  Immunocytochemistry - Anti-SMUG1 antibody (ab15716)

  Immunocytochemistry/immunofluorescence analysis of U2OS cells labelling SMUG1 with ab15716 at 10 µg/mL showing strong nuclear staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). Nuclear DNA was labelled with DAPI (blue).

  Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).

• 

  Immunocytochemistry - Anti-SMUG1 antibody (ab15716)

  Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling SMUG1 with ab15716 at 10 µg/mL showing strong nuclear and cytoplasmic staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). Nuclear DNA was labelled with DAPI (blue).

  Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).