Anti-ACE2抗体
参阅全部 ACE2 一抗
Immunogen affinity purified
No cross reactivity to ACE1.
多克隆
IgG
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Unigene: 13451 Mouse
Unigene: 129779 Rat
Entrez Gene: 59272 Human
Entrez Gene: 70008 Mouse
Omim: 300335 Human
SwissProt: Q9BYF1 Human
SwissProt: Q8R0I0 Mouse
Unigene: 178098 Human
ACE2 antibody
ACE2_HUMAN antibody
ACEH antibody
Angiotensin converting enzyme 2 antibody
Angiotensin converting enzyme homolog antibody
Angiotensin converting enzyme like protein antibody
Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
Angiotensin I converting enzyme 2 antibody
Angiotensin-converting enzyme homolog antibody
DKFZP434A014 antibody
EC 3.4.17 antibody
metalloprotease MPROT 15 antibody
Metalloprotease MPROT15 antibody
OTTHUMP00000022963 antibody
Processed angiotensin-converting enzyme 2 antibody
ACE 2 antibody
ACE related carboxypeptidase antibody
ACE-related carboxypeptidase antibody
Rabbit
No cross reactivity to ACE1.
适用于: ICC/IF, ELISA, IHC (PFA fixed), IHC-Fr, WB, IHC-Pmore details
与反应: Mouse, Rat, Cat, Human, Ferret, Macaque monkey, Bat
Synthetic peptide within Human ACE2 aa 750 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9BYF1
WB: Human testis, lung, intestine, breast and kidney tissue lysates. Caco2 cell lysate. Rat skin, spleen, heart and brain tissue lysates. Mouse liver, spleen, heart, bladder, pancreas and stomach tissue lysates. IHC-P: Human lung, testis and kidney tissue. Rat lung tissue. Mouse lung tissue.
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If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C.
pH: 7.2
Preservative: 0.02% Sodium azide
浓度
100 µg 浓度为 1 mg/ml
Western blot - Anti-ACE2 antibody (ab15348)
All lanes : Anti-ACE2 antibody (ab15348) at 1/1000 dilution
Lane 1 : Wild-type Caco-2 cell lysate
Lane 2 : ACE2 knockout Caco-2 cell lysate
Lane 3 : Calu-3 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 120-135 kDawhy is the actual band size different from the predicted?
False colour image of Western blot: Anti-ACE2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab15348 was shown to bind specifically to ACE2. A band was observed at 120-135 kDa in wild-type Caco-2 cell lysates with no signal observed at this size in ACE2 knockout cell line ab277338 (knockout cell lysate ab275516). To generate this image, wild-type and ACE2 knockout Caco-2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Western blot - Anti-ACE2 antibody (ab15348)
All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Human Testis Lysate
Lane 2 : Human Lung lysate
Lane 3 : Human intestine lysate
Lane 4 : Human Breast lysate
Lane 5 : Caco2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 125 kDawhy is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Western blot - Anti-ACE2 antibody (ab15348)
All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Rat skin tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat heart tissue lysate
Lane 4 : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 125 kDawhy is the actual band size different from the predicted?
Western blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.
Western blot - Anti-ACE2 antibody (ab15348)
All lanes : Anti-ACE2 antibody (ab15348) at 2 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse bladder tissue lysate
Lane 5 : Mouse pancreas tissue lysate
Lane 6 : Mouse stomach tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 97 kDa
Observed band size: 110 kDawhy is the actual band size different from the predicted?
Western blot of ab15348 incubated for 1h at RT in 5% NFDM/TBST.
Western blot - Anti-ACE2 antibody (ab15348)
Lane 1 : Anti-ACE2 antibody (ab15348) at 0.5 µg/ml
Lane 2 : Anti-ACE2 antibody (ab15348) at 1 µg/ml
Lane 3 : Anti-ACE2 antibody (ab15348) at 2 µg/ml
All lanes : Human Kidney Lysate
Predicted band size: 97 kDa
Observed band size: 105 kDawhy is the actual band size different from the predicted?
Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5 μg/mL (lane A), 1 μg/mL (lane B), and 2 μg/mL (lane C).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labeling ACE2 with ab15348 at 20 μg/mL.
Goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody (ab15348)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling ACE2 with ab15348 at 2 μg/mL.
Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG HRP at 1/250 was used as secondary. Counter stained with Hematoxylin.
Abpromise™承诺保证使用ab15348于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) | Use at an assay dependent concentration. |
| ELISA | Use at an assay dependent concentration. | |
| IHC (PFA fixed) | Use at an assay dependent concentration. | |
| IHC-Fr | (2) | Use at an assay dependent concentration. |
| WB | (5) | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). |
| IHC-P | (5) | Use a concentration of 2 µg/ml. |
