Anti-LKB1 antibody [Ley 37D/G6]

• 产品名称

  Anti-LKB1抗体[Ley 37D/G6]
  参阅全部 LKB1 一抗

• 描述

  小鼠单克隆抗体[Ley 37D/G6] to LKB1

• 宿主

  Mouse

• 特异性

  This antibody detects a single clean band representing LKB1/STK11 in Western blots on cells expressing LKB1.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant full length protein corresponding to Human LKB1.
  Database link: Q15831

• 阳性对照

• HAP1 and MEF cell lysates.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C. Do Not Freeze.

• 存储溶液

  pH: 7.4
  Preservative: 0.02% Sodium azide
  Constituents: 1% BSA, PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 克隆

  单克隆

• 克隆编号

  Ley 37D/G6

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab15095于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 6794 Human

• Entrez Gene: 20869 Mouse

• Omim: 602216 Human

• SwissProt: Q15831 Human

• SwissProt: Q9WTK7 Mouse

• Unigene: 515005 Human

• 别名

• PJS antibody

• Polarization related protein LKB1 antibody

• Renal carcinoma antigen NY-REN-19 antibody

• Serine/Threonine Kinase 11 antibody

• Serine/threonine protein kinase 11 antibody

• Serine/threonine protein kinase LKB1 antibody

• Serine/threonine protein kinase STK11 antibody

• Serine/threonine-protein kinase 11 antibody

• Serine/threonine-protein kinase LKB1 antibody

• Serine/threonine-protein kinase XEEK1 antibody

• Stk11 antibody

• STK11_HUMAN antibody

• hLKB1 antibody

• Liver kinase B1 antibody

• LKB1 antibody

• 

  Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: LKB1 knockout HAP1 cell lysate (20 µg)

  Lanes 1 and 2: Merged signal (red and green). Green - ab15095 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
  ab15095 was shown to recognize LKB1 when LKB1 knockout samples were used, along with additional cross-reactive bands. Wild-type and LKB1 knockout samples were subjected to SDS-PAGE. ab15095 and ab181602 (loading control to LKB1) were diluted 1/100 and 1/10 000 and incubated overnight at 4°C. Blots were developed withGoat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

• 

  Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)Image courtesy of anonymous abreviewer

  All lanes : Anti-LKB1 antibody [Ley 37D/G6] (ab15095) at 1/1000 dilution
  
  All lanes :
  
  Secondary
  All lanes : Polyclonal goat anti-mouse conjugated with horse radish peroxidase.
  
  Predicted band size: 48.6 kDa
  Observed band size: 55 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Negative control (Lane 1): LKB1 knockout MEF cells.

  Positive control (Lane 2): wildtype MEF cells.

  Blocking step: 5% milk as blocking agent for 1 hour at 25 °C.

  Incubation: 16 hours at 4 °C, using 5% milk in TBS/0.1 Tween as diluent.

   

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