Anti-Carbonic Anhydrase 9/CA9抗体
参阅全部 Carbonic Anhydrase 9/CA9 一抗
兔多克隆抗体to Carbonic Anhydrase 9/CA9
Rabbit
适用于: Flow Cyt (Intra), ICC, IHC-Fr, IHC-P, WBmore details
与反应: Rat, Human
Synthetic peptide corresponding to Human Carbonic Anhydrase 9/CA9 aa 359-459.
Sequence:
LSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNS CLAAGDILALVFGLLFAVTSVAFLVQMRRQHRRGTKGGVSYRPAEVAETG A
WB: Ramos, A549, HeLa and MDA-MB-231 cell lysates; Rat renal cortex lysate. Intr Flow Cyt: A-431 and U-87 MG cells. ICC: A-431 cells. IHC-Fr: Human renal cell cancer tumor sections. IHC-P: Human breast cancer sections.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.1% Sodium azide
浓度
100 µl 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15086于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) | 1/1000. | |
| ICC | Use a concentration of 2 - 5 µg/ml. | |
| IHC-Fr | (1) | 1/200 - 1/500. |
| IHC-P | (2) | 1/200 - 1/500. |
| WB | (1) | Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49.7 kDa).Can be blocked with Recombinant Human RND1 protein (ab109840). |
Entrez Gene: 768 Human
Omim: 603179 Human
SwissProt: Q16790 Human
Unigene: 63287 Human
CAIX antibody
Carbonate dehydratase IX antibody
Carbonic anhydrase 9 antibody
Carbonic anhydrase IX antibody
Carbonic dehydratase antibody
G250 antibody
Membrane antigen MN antibody
MN antibody
P54/58N antibody
pMW1 antibody
RCC associated protein G250 antibody
RCC-associated antigen G250 antibody
Renal cell carcinoma-associated antigen G250 antibody
CA-IX antibody
CA9 antibody
CAH9_HUMAN antibody
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)
Western blot analysis labeling Carbonic Anhydrase 9/CA9 with ab15086 at 1 ug/ml in rat renal cortex lysate.
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)
Western blot analysis labeling Carbonic Anhydrase 9/CA9 with ab15086 at 1 ug/ml in 1) HeLa, 2) MDA-MB-231, and 3) A549 whole cell lysates.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)
Immunohistochemistry analysis of a formalin-fixed, paraffin-embedded tissue section of human breast cancer using ab15086 at 1:1000 dilution. The primary antibody bound to Carbonic Anydrase 9/CA9 antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This antibody generated an expected cytoplasmic staining of Carbonic Anydrase 9/CA9 protein with an intense signal around the cellular membranes in tumor cores. The latter are more likely to be hypoxic in growing tumors which signifies that the observed Carbonic Anydrase 9/CA9 staining is specific.
Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)
Immunohistochemistry analysis of human renal cell cancer tumor cryosections stained with ab15086 at a 1/500 dilution (left) or normal rabbit serum (right).
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)
All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086) at 1 µg/ml
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 49.7 kDa
Observed band size: 55 kDawhy is the actual band size different from the predicted?
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