Anti-SOCS3抗体[SO1]
参阅全部 SOCS3 一抗
小鼠单克隆抗体[SO1] to SOCS3
Mouse
适用于: Flow Cyt, ELISA, WB, ICC/IFmore details
与反应: Rat, Human
Full length native protein (purified) (Human).
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
Protein A purified
单克隆
SO1
IgG2b
Abpromise™承诺保证使用ab14939于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | (2) | Use at an assay dependent concentration. |
| ICC/IF | Use a concentration of 5 µg/ml. |
Entrez Gene: 9021 Human
Omim: 604176 Human
SwissProt: O14543 Human
Unigene: 527973 Human
Unigene: 127801 Rat
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![Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [SO1] (ab14939)](https://www.abcam.cn/ps/products/14/ab14939/Images/ab14939-22206-anti-socs3-antibody-so1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [SO1] (ab14939)
ICC/IF image of ab14939 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Flow Cytometry - Anti-SOCS3 antibody [SO1] (ab14939)](https://www.abcam.cn/ps/products/14/ab14939/Images/ab14939-22205-anti-socs3-antibody-so1-flow-cytometry.jpg)
Flow Cytometry - Anti-SOCS3 antibody [SO1] (ab14939)
Overlay histogram showing Jurkat cells stained with ab14939 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14939, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
