Anti-PPM1A antibody [p6c7]

• 产品名称

  Anti-PPM1A抗体[p6c7]
  参阅全部 PPM1A 一抗

• 描述

  小鼠单克隆抗体[p6c7] to PPM1A

• 宿主

  Mouse

• 经测试应用

  适用于: ELISA, IHC-P, WB, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant full length protein (Human).

• 阳性对照

• WB: HeLa, HAP1, Jurkat, K562, MCF7, A549 and Raji cell lysates; Mouse kidney, brain and liver lysates; Mouse liver cytosol extract. ICC: HeLa cells.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 28/02/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 10% Glycerol

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  p6c7

• 骨髓瘤

  Sp2/0

• 同种型

  IgG2b

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab14824于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5494 Human

• Entrez Gene: 19042 Mouse

• Omim: 606108 Human

• SwissProt: P35813 Human

• SwissProt: P49443 Mouse

• Unigene: 130036 Human

• Unigene: 261045 Mouse

• 别名

• IA antibody

• MGC9201 antibody

• Mpp alpha antibody

• PP2C alpha antibody

• PP2C-alpha antibody

• PP2CA antibody

• PP2Calpha antibody

• PPM 1A antibody

• PPM1A antibody

• PPM1A_HUMAN antibody

• PPPM1A antibody

• Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform antibody

• Protein phosphatase 1A antibody

• Protein phosphatase 1A magnesium dependent alpha antibody

• Protein phosphatase 2C alpha antibody

• Protein phosphatase 2C alpha isoform antibody

• Protein phosphatase 2C isoform alpha antibody

• Protein phosphatase IA antibody

• Protein phosphatase, Mg2+/Mn2+ dependent, 1A antibody

• Protein phosphatase 2C isoform alpha antibody

• EC 3.1.3.16 antibody

• FLJ42306 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-PPM1A antibody [p6c7] (ab14824)

  Immunocytochemistry/ Immunofluorescence analysis of PP2C alpha/PPM1A in HeLa cells. The cell was stained with ab14824 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

• 

  Western blot - Anti-PPM1A antibody [p6c7] (ab14824)

  All lanes : Anti-PPM1A antibody [p6c7] (ab14824) at 1/500 dilution
  
  Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  Lane 2 : PPM1A knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  Lane 3 : HAP1 whole cell lyate
  Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
  
  Predicted band size: 42 kDa
  Observed band size: 42 kDa
  
  
  
  

  Lanes 1-4: Merged signal (red and green). Green - ab14824 observed at 42 kDa. Red - loading control ab181602 observed at 36 kDa.

   ab14824 Anti-PPM1A antibody [p6c7]  was shown to specifically react with PPM1A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265348 (knockout cell lysate ab259055) was used. Wild-type and PPM1A knockout samples were subjected to SDS-PAGE. ab14824 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

• 

  Western blot - Anti-PPM1A antibody [p6c7] (ab14824)

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: PPM1A knockout HAP1 cell lysate (20 µg)
  Lane 3: HeLa cell lysate (20 µg)
  Lane 4: Jurkat cell lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab14824 observed at 42 kDa. Red - loading control, ab18251, observed at 52 kDa.
  
  ab14824 was shown to specifically react with PPM1A when PPM1A knockout samples were used. Wild-type and PPM1A knockout samples were subjected to SDS-PAGE. ab14824 diluted to 1/250 and ab18251 (loading control to alpha Tubulin) diluted to 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD)  preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  This image was generated using the ascites version of the product.

• 

  Western blot - Anti-PPM1A antibody [p6c7] (ab14824)

  All lanes : Anti-PPM1A antibody [p6c7] (ab14824) at 1/1000 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : HeLa cell lysate
  Lane 3 : K-562 cell lysate
  Lane 4 : MCF7 cell lysate
  Lane 5 : A549 cell lysate
  Lane 6 : Raji cell lysate
  Lane 7 : Mouse kidney tissue lysate
  Lane 8 : Mouse brain tissue lysate
  Lane 9 : Mouse liver tissue lysate
  
  Lysates/proteins at 40 µg per lane.
  
  Secondary
  All lanes : goat anti-mouse secondary antibody conjugated to HRP
  
  Predicted band size: 42 kDa
  
  

• 

  Western blot - Anti-PPM1A antibody [p6c7] (ab14824)

  Western blot analysis of mouse liver cytosol extract using ab14824 at a dilution of 1/250.  Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system. Western blot analysis of mouse liver cytosol extract using ab14824 at a dilution of 1/250. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.

  This image was generated using the ascites version of the product.

• 

  Western blot - Anti-PPM1A antibody [p6c7] (ab14824)

  Anti-PPM1A antibody [p6c7] (ab14824) at 1/1000 dilution + HeLa whole cell lysate
  
  Secondary
  HRP conjugated anti-mouse antibody
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 42 kDa
  Observed band size: 45 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 10 seconds
  
  
  

  This image is courtesy of an Abreview submitted by Xia Lin on 2 March 2006.

  This image was generated using the ascites version of the product.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1A antibody [p6c7] (ab14824)

  Ab14824 staining human colon. Staining is localised to cytoplasm.
  Left panel: with primary antibody at 4ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  This image was generated using the ascites version of the product.